Block of the α9 nicotinic receptor by ototoxic aminoglycosides
In the present study, we report that the α9 nicotinic acetylcholine receptor (nAChR) expressed in Xenopus laevis oocytes is reversibly blocked by aminoglycoside antibiotics. The aminoglycosides tested blocked the α9 nAChR in a concentration-dependent manner with the following rank order of potency:...
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| Autores principales: | , |
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| Publicado: |
2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00283908_v39_n13_p2525_Rothlin http://hdl.handle.net/20.500.12110/paper_00283908_v39_n13_p2525_Rothlin |
| Aporte de: |
| Sumario: | In the present study, we report that the α9 nicotinic acetylcholine receptor (nAChR) expressed in Xenopus laevis oocytes is reversibly blocked by aminoglycoside antibiotics. The aminoglycosides tested blocked the α9 nAChR in a concentration-dependent manner with the following rank order of potency: neomycin>gentamicin>streptomycin>amikacin>kanamycin. The antagonistic effect of gentamicin was not overcome by increasing the concentration of acetylcholine (ACh), indicative of a non-competitive type of block. Blockage of ACh-evoked currents by gentamicin was found to be voltage-dependent, being more potent at hyperpolarized than at depolarized holding potentials. Furthermore, gentamicin blockage was dependent upon the extracellular Ca2+ concentration, shown by the fact that increments in extracellular Ca2+ significantly reduced the potency of this aminoglycoside to block the α9 nAChR. Possible mechanisms of blockage by the aminoglycosides are discussed. The present results suggest that the initial reversible actions of aminoglycosides at the organ of Corti, such as the elimination of the olivocochlear efferent function, are due in part to the interaction with the native α9-containing cholinergic receptor of the outer hair cells. Copyright (C) 2000 Elsevier Science Ltd. |
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