Antibodies against epididymal glycoproteins block fertilizing ability in rat

Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protei...

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Guardado en:
Detalles Bibliográficos
Publicado: 1984
Materias:
antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v72_n2_p467_Cuasnicu
http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00224251_v72_n2_p467_Cuasnicu
record_format dspace
spelling paper:paper_00224251_v72_n2_p467_Cuasnicu2023-06-08T14:50:53Z Antibodies against epididymal glycoproteins block fertilizing ability in rat antibody glycoprotein sperm antibody animal model epididymis fertilization male genital system nonhuman priority journal rat Animalia Oryctolagus cuniculus Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protein DE fertilized only 6.6% (P < 0.01). An equal amount of normal rabbit serum (NRS) did not cause inhibition (33.1%). To study the entry of antibodies into the epididymis, caudal tubules were cultured for 24 h and the fertility of the contained spermatozoa was assessed by artificial insemination. Culture in Medium 199 alone or with NRS resulted in spermatozoa which fertilized 52% of oocytes while the presence of antiserum to protein DE in the culture medium yielded spermatozoa which fertilized only 16.6% of oocytes (P < 0.01). These results suggest (1) that the epididymal protein DE might be part of a sperm structure involved in the fertilization process, and (2) that, at least under the present culture conditions, immunoglobulins penetrate the epididymal epithelium in sufficient numbers to reduce fertility significantly. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v72_n2_p467_Cuasnicu http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
spellingShingle antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
Antibodies against epididymal glycoproteins block fertilizing ability in rat
topic_facet antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
description Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protein DE fertilized only 6.6% (P < 0.01). An equal amount of normal rabbit serum (NRS) did not cause inhibition (33.1%). To study the entry of antibodies into the epididymis, caudal tubules were cultured for 24 h and the fertility of the contained spermatozoa was assessed by artificial insemination. Culture in Medium 199 alone or with NRS resulted in spermatozoa which fertilized 52% of oocytes while the presence of antiserum to protein DE in the culture medium yielded spermatozoa which fertilized only 16.6% of oocytes (P < 0.01). These results suggest (1) that the epididymal protein DE might be part of a sperm structure involved in the fertilization process, and (2) that, at least under the present culture conditions, immunoglobulins penetrate the epididymal epithelium in sufficient numbers to reduce fertility significantly.
title Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_short Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_full Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_fullStr Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_full_unstemmed Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_sort antibodies against epididymal glycoproteins block fertilizing ability in rat
publishDate 1984
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v72_n2_p467_Cuasnicu
http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
_version_ 1768541595476426752

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