Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro
The fertility of spermatozoa from the different epididymal segments of hamsters was tested by in-vivo insemination. Caput and proximal corpus spermatozoa were non-fertile; spermatozoa from the distal corpus epididymidis fertilized 13% (38/290) oocytes and those from the proximal and distal cauda epi...
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1984
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p433_GonzalezEcheverria |
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paper:paper_00224251_v71_n2_p433_GonzalezEcheverria |
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paper:paper_00224251_v71_n2_p433_GonzalezEcheverria2023-06-08T14:50:52Z Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro chorionic gonadotropin animal experiment drug efficacy epididymis epididymis protein fertilization gonadotropin blood level in vitro study male genital system nonhuman spermatozoon Animal Epididymis Female Fertilization Fertilization in Vitro Glycoproteins Hamsters Male Mesocricetus Sperm Maturation Tissue Extracts The fertility of spermatozoa from the different epididymal segments of hamsters was tested by in-vivo insemination. Caput and proximal corpus spermatozoa were non-fertile; spermatozoa from the distal corpus epididymidis fertilized 13% (38/290) oocytes and those from the proximal and distal cauda epididymidis 71 and 87%, respectively. When tested by in-vitro insemination, distal corpus spermatozoa penetrated 44% of oocytes while those from the distal cauda fertilized 87% of oocytes. Spermatozoa from the distal corpus recovered in Medium BMOC fertilized 13% (28/210) of oocytes in vivo, while those mixed with an epididymal protein preparation (0.8 mg protein/ml) fertilized 24% (49/204; P<0.01) of oocytes. When distal corpus spermatozoa were inseminated in vivo with 0.8 mg epididymal protein preparation 34% (31/90) oocytes were fertilized and only 22% (23/103; P<0.05) oocytes were fertilized when the proteins were obtained from epididymides of animals castrated for 30 days. When distal corpus spermatozoa were preincubated for 5 h in medium without (control) or with protein preparation (0.8 or 1.6 mg protein/ml), a significant increase in in-vitro oocyte penetration was found (25 compared with 45%; P<0.05) when the protein was present at 1.6 mg/ml. These results confirm and extend previous observations suggesting a role for androgen-dependent glycoproteins secreted by the epididymis in the acquisition of fertilizing ability that occurs during sperm maturation. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p433_GonzalezEcheverria |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
chorionic gonadotropin animal experiment drug efficacy epididymis epididymis protein fertilization gonadotropin blood level in vitro study male genital system nonhuman spermatozoon Animal Epididymis Female Fertilization Fertilization in Vitro Glycoproteins Hamsters Male Mesocricetus Sperm Maturation Tissue Extracts |
| spellingShingle |
chorionic gonadotropin animal experiment drug efficacy epididymis epididymis protein fertilization gonadotropin blood level in vitro study male genital system nonhuman spermatozoon Animal Epididymis Female Fertilization Fertilization in Vitro Glycoproteins Hamsters Male Mesocricetus Sperm Maturation Tissue Extracts Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| topic_facet |
chorionic gonadotropin animal experiment drug efficacy epididymis epididymis protein fertilization gonadotropin blood level in vitro study male genital system nonhuman spermatozoon Animal Epididymis Female Fertilization Fertilization in Vitro Glycoproteins Hamsters Male Mesocricetus Sperm Maturation Tissue Extracts |
| description |
The fertility of spermatozoa from the different epididymal segments of hamsters was tested by in-vivo insemination. Caput and proximal corpus spermatozoa were non-fertile; spermatozoa from the distal corpus epididymidis fertilized 13% (38/290) oocytes and those from the proximal and distal cauda epididymidis 71 and 87%, respectively. When tested by in-vitro insemination, distal corpus spermatozoa penetrated 44% of oocytes while those from the distal cauda fertilized 87% of oocytes. Spermatozoa from the distal corpus recovered in Medium BMOC fertilized 13% (28/210) of oocytes in vivo, while those mixed with an epididymal protein preparation (0.8 mg protein/ml) fertilized 24% (49/204; P<0.01) of oocytes. When distal corpus spermatozoa were inseminated in vivo with 0.8 mg epididymal protein preparation 34% (31/90) oocytes were fertilized and only 22% (23/103; P<0.05) oocytes were fertilized when the proteins were obtained from epididymides of animals castrated for 30 days. When distal corpus spermatozoa were preincubated for 5 h in medium without (control) or with protein preparation (0.8 or 1.6 mg protein/ml), a significant increase in in-vitro oocyte penetration was found (25 compared with 45%; P<0.05) when the protein was present at 1.6 mg/ml. These results confirm and extend previous observations suggesting a role for androgen-dependent glycoproteins secreted by the epididymis in the acquisition of fertilizing ability that occurs during sperm maturation. |
| title |
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| title_short |
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| title_full |
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| title_fullStr |
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| title_full_unstemmed |
Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| title_sort |
addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro |
| publishDate |
1984 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p433_GonzalezEcheverria http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p433_GonzalezEcheverria |
| _version_ |
1768542774063267840 |