Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa
The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were in...
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1984
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00224251_v71_n2_p427_Cuasnicu http://hdl.handle.net/20.500.12110/paper_00224251_v71_n2_p427_Cuasnicu |
| Aporte de: |
| Sumario: | The increase in zona pellucida binding caused by the exposure of cultured proximal corpus epididymidis to 2 μM-5α-DHT (0.87 and 4.20 spermatozoa/egg for control and 5α-DHT group respectively) was lost when 20 μM-cycloheximide was also added to the medium (0.72 spermatozoa/egg). These results were interpreted as meaning that de-novo protein synthesis was required to obtain the effect of androgens. When a fraction enriched in epididymal glycoproteins (EP2-EP6 (18% total protein in epididymal cytosol and 30% in enriched fraction) and depleted of androgens (<120 pg testosterone + DHT/ml) was added to the cultured epididymal tubules, the zona pellucida-binding ability of the contained spermatozoa increased from 0.55 in controls to 2.73 spermatozoa/egg in the extract-treated group (P<0.02). When the enriched fraction was prepared from epididymides of 30-day castrates, the stimulatory effect was lost (1.04 spermatozoa/egg). We suggest that proteins synthesized in the epididymis are required to obtain the effect of androgens and that the glycoproteins EP2-EP6 may be involved. |
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