Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas
Curcumin (diferuloylmethane), a polyphenolic compound derived from the spice plant Curcuma longa, displays multiple actions on solid tumours including anti-angiogenic effects. Here we have studied in rodent and human pituitary tumour cells the influence of curcumin on the production of hypoxia induc...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v214_n3_p389_Shan http://hdl.handle.net/20.500.12110/paper_00220795_v214_n3_p389_Shan |
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paper:paper_00220795_v214_n3_p389_Shan2023-06-08T14:45:28Z Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas curcumin hypoxia inducible factor 1alpha messenger RNA vasculotropin vasculotropin A adult aged angiogenesis animal cell article female human human cell hypophysis adenoma in vitro study male nonhuman priority journal protein secretion protein synthesis rat reverse transcription polymerase chain reaction Western blotting Adenoma Animals Antineoplastic Agents Cell Hypoxia Cell Line, Tumor Corticotrophs Curcumin Humans Hypoxia-Inducible Factor 1, alpha Subunit Lactotrophs Mice Neovascularization, Pathologic Pituitary Neoplasms Rats RNA, Messenger Somatotrophs Vascular Endothelial Growth Factor A Curcumin (diferuloylmethane), a polyphenolic compound derived from the spice plant Curcuma longa, displays multiple actions on solid tumours including anti-angiogenic effects. Here we have studied in rodent and human pituitary tumour cells the influence of curcumin on the production of hypoxia inducible factor 1α (HIF1A) and vascular endothelial growth factor A (VEGFA), two key components involved in tumour neovascularisation through angiogenesis. Curcumin dose-dependently inhibited basal VEGFA secretion in corticotroph AtT20 mouse and lactosomatotroph GH3 rat pituitary tumour cells as well as in all human pituitary adenoma cell cultures (nZ32) studied. Under hypoxia-mimicking conditions (CoCl 2 treatment) in AtT20 and GH3 cells as well as in all human pituitary adenoma cell cultures (nZ8) studied, curcumin strongly suppressed the induction of mRNA synthesis and protein production of HIF1A, the regulated subunit of the hypoxia-induced transcription factor HIF1. Curcumin also blocked hypoxiainducedmRNAsynthesis and secretion ofVEGFAinGH3 cells and in all human pituitary adenoma cell cultures investigated (nZ18). Thus, curcumin may inhibit pituitary adenoma progression not only through previously demonstrated antiproliferative and pro-apoptotic actions but also by its suppressive effects on pituitary tumour neovascularisation. © 2012 Society for Endocrinology. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v214_n3_p389_Shan http://hdl.handle.net/20.500.12110/paper_00220795_v214_n3_p389_Shan |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
curcumin hypoxia inducible factor 1alpha messenger RNA vasculotropin vasculotropin A adult aged angiogenesis animal cell article female human human cell hypophysis adenoma in vitro study male nonhuman priority journal protein secretion protein synthesis rat reverse transcription polymerase chain reaction Western blotting Adenoma Animals Antineoplastic Agents Cell Hypoxia Cell Line, Tumor Corticotrophs Curcumin Humans Hypoxia-Inducible Factor 1, alpha Subunit Lactotrophs Mice Neovascularization, Pathologic Pituitary Neoplasms Rats RNA, Messenger Somatotrophs Vascular Endothelial Growth Factor A |
| spellingShingle |
curcumin hypoxia inducible factor 1alpha messenger RNA vasculotropin vasculotropin A adult aged angiogenesis animal cell article female human human cell hypophysis adenoma in vitro study male nonhuman priority journal protein secretion protein synthesis rat reverse transcription polymerase chain reaction Western blotting Adenoma Animals Antineoplastic Agents Cell Hypoxia Cell Line, Tumor Corticotrophs Curcumin Humans Hypoxia-Inducible Factor 1, alpha Subunit Lactotrophs Mice Neovascularization, Pathologic Pituitary Neoplasms Rats RNA, Messenger Somatotrophs Vascular Endothelial Growth Factor A Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| topic_facet |
curcumin hypoxia inducible factor 1alpha messenger RNA vasculotropin vasculotropin A adult aged angiogenesis animal cell article female human human cell hypophysis adenoma in vitro study male nonhuman priority journal protein secretion protein synthesis rat reverse transcription polymerase chain reaction Western blotting Adenoma Animals Antineoplastic Agents Cell Hypoxia Cell Line, Tumor Corticotrophs Curcumin Humans Hypoxia-Inducible Factor 1, alpha Subunit Lactotrophs Mice Neovascularization, Pathologic Pituitary Neoplasms Rats RNA, Messenger Somatotrophs Vascular Endothelial Growth Factor A |
| description |
Curcumin (diferuloylmethane), a polyphenolic compound derived from the spice plant Curcuma longa, displays multiple actions on solid tumours including anti-angiogenic effects. Here we have studied in rodent and human pituitary tumour cells the influence of curcumin on the production of hypoxia inducible factor 1α (HIF1A) and vascular endothelial growth factor A (VEGFA), two key components involved in tumour neovascularisation through angiogenesis. Curcumin dose-dependently inhibited basal VEGFA secretion in corticotroph AtT20 mouse and lactosomatotroph GH3 rat pituitary tumour cells as well as in all human pituitary adenoma cell cultures (nZ32) studied. Under hypoxia-mimicking conditions (CoCl 2 treatment) in AtT20 and GH3 cells as well as in all human pituitary adenoma cell cultures (nZ8) studied, curcumin strongly suppressed the induction of mRNA synthesis and protein production of HIF1A, the regulated subunit of the hypoxia-induced transcription factor HIF1. Curcumin also blocked hypoxiainducedmRNAsynthesis and secretion ofVEGFAinGH3 cells and in all human pituitary adenoma cell cultures investigated (nZ18). Thus, curcumin may inhibit pituitary adenoma progression not only through previously demonstrated antiproliferative and pro-apoptotic actions but also by its suppressive effects on pituitary tumour neovascularisation. © 2012 Society for Endocrinology. |
| title |
Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| title_short |
Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| title_full |
Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| title_fullStr |
Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| title_full_unstemmed |
Curcumin suppresses HIF1A synthesis and VEGFA release in pituitary adenomas |
| title_sort |
curcumin suppresses hif1a synthesis and vegfa release in pituitary adenomas |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00220795_v214_n3_p389_Shan http://hdl.handle.net/20.500.12110/paper_00220795_v214_n3_p389_Shan |
| _version_ |
1768546381978402816 |