The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway

The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	2009
Materias:	Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n14_p2453_Mukherjee http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00219533_v122_n14_p2453_Mukherjee
record_format	dspace
spelling	paper:paper_00219533_v122_n14_p2453_Mukherjee2023-06-08T14:43:42Z The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated that the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present evidence that yeast epsin 2 (Ent2) plays a signaling role during cell division. We observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1, promoted the formation of chains of cells and aberrant septa. This dominant-negative effect resulted from ENTH2-mediated interference with septin assembly pathways. We mapped the ENTH2 determinants responsible for induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth and cytokinesis and rescued a defect in cell division induced by Bem3 misregulation. Collectively, our findings provide a potential molecular mechanism linking endocytosis (via epsin 2) with signaling pathways regulating cell division. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n14_p2453_Mukherjee http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins
spellingShingle	Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
topic_facet	Cell division Endocytosis Epsin Septin article error priority journal Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Cell Division Cell Polarity Chitin Synthase Cytokinesis Endocytosis Gene Expression Regulation, Fungal Genotype GTPase-Activating Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Phenotype Protein Structure, Tertiary Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Signal Transduction Vesicular Transport Proteins
description	The epsins are a family of adaptors involved in recruiting other endocytic proteins, binding of ubiquitylated cargo and induction of membrane curvature. These molecules bear a characteristic epsin N-terminal homology (ENTH) domain and multiple peptide motifs that mediate protein-protein interactions. We have previously demonstrated that the ENTH domain of epsin is involved in Cdc42 signaling regulation. Here, we present evidence that yeast epsin 2 (Ent2) plays a signaling role during cell division. We observed that overexpression of the ENTH domain of Ent2 (ENTH2), but not Ent1, promoted the formation of chains of cells and aberrant septa. This dominant-negative effect resulted from ENTH2-mediated interference with septin assembly pathways. We mapped the ENTH2 determinants responsible for induction of the phenotype and found them to be important for efficient binding to the septin regulatory protein, Bem3. Supporting a physiological role for epsin 2 in cell division, the protein localized to sites of polarized growth and cytokinesis and rescued a defect in cell division induced by Bem3 misregulation. Collectively, our findings provide a potential molecular mechanism linking endocytosis (via epsin 2) with signaling pathways regulating cell division.
title	The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
title_short	The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
title_full	The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
title_fullStr	The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
title_full_unstemmed	The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway
title_sort	yeast endocytic protein epsin 2 functions in a cell-division signaling pathway
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219533_v122_n14_p2453_Mukherjee http://hdl.handle.net/20.500.12110/paper_00219533_v122_n14_p2453_Mukherjee
_version_	1768545587600293888

The yeast endocytic protein Epsin 2 functions in a cell-division signaling pathway

Ejemplares similares