Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation
Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which we have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and a...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219525_v195_n7_p1141_Baez http://hdl.handle.net/20.500.12110/paper_00219525_v195_n7_p1141_Baez |
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paper:paper_00219525_v195_n7_p1141_Baez2023-06-08T14:43:40Z Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation Baez, María Verónica Luchelli, Luciana Maschi, Darío Habif, Martín Thomas, María Gabriela Boccaccio, Graciela Lidia biological factor messenger RNA n methyl dextro aspartic acid n methyl dextro aspartic acid receptor smaug1 protein unclassified drug article cell aggregation cell count cell membrane depolarization cell size controlled study female gene silencing hippocampus human human cell nerve cell plasticity priority journal protein expression RNA binding RNA translation synapse synaptogenesis Mammalia Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which we have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and are the first described mRNA-silencing foci specific to neurons. RNA binding was not required for aggregation, which indicates that S-foci formation is not a consequence of mRNA silencing. N-methyl-D-aspartic acid (NMDA) receptor stimulation provoked a rapid and reversible disassembly of S-foci, transiently releasing transcripts (the CaMKIIα mRNA among others) to allow their translation. Simultaneously, NMDA triggered global translational silencing, which suggests the specific activation of Smaug1-repressed transcripts. Smaug1 is expressed during synaptogenesis, and Smaug1 knockdown affected the number and size of synapses, and also provoked an impaired response to repetitive depolarizing stimuli, as indicated by a reduced induction of Arc/Arg3.1. Our results suggest that S-foci control local translation, specifically responding to NMDA receptor stimulation and affecting synaptic plasticity. © 2011 Baez et al. Fil:Baez, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luchelli, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maschi, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Habif, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Thomas, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219525_v195_n7_p1141_Baez http://hdl.handle.net/20.500.12110/paper_00219525_v195_n7_p1141_Baez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
biological factor messenger RNA n methyl dextro aspartic acid n methyl dextro aspartic acid receptor smaug1 protein unclassified drug article cell aggregation cell count cell membrane depolarization cell size controlled study female gene silencing hippocampus human human cell nerve cell plasticity priority journal protein expression RNA binding RNA translation synapse synaptogenesis Mammalia |
| spellingShingle |
biological factor messenger RNA n methyl dextro aspartic acid n methyl dextro aspartic acid receptor smaug1 protein unclassified drug article cell aggregation cell count cell membrane depolarization cell size controlled study female gene silencing hippocampus human human cell nerve cell plasticity priority journal protein expression RNA binding RNA translation synapse synaptogenesis Mammalia Baez, María Verónica Luchelli, Luciana Maschi, Darío Habif, Martín Thomas, María Gabriela Boccaccio, Graciela Lidia Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| topic_facet |
biological factor messenger RNA n methyl dextro aspartic acid n methyl dextro aspartic acid receptor smaug1 protein unclassified drug article cell aggregation cell count cell membrane depolarization cell size controlled study female gene silencing hippocampus human human cell nerve cell plasticity priority journal protein expression RNA binding RNA translation synapse synaptogenesis Mammalia |
| description |
Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which we have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and are the first described mRNA-silencing foci specific to neurons. RNA binding was not required for aggregation, which indicates that S-foci formation is not a consequence of mRNA silencing. N-methyl-D-aspartic acid (NMDA) receptor stimulation provoked a rapid and reversible disassembly of S-foci, transiently releasing transcripts (the CaMKIIα mRNA among others) to allow their translation. Simultaneously, NMDA triggered global translational silencing, which suggests the specific activation of Smaug1-repressed transcripts. Smaug1 is expressed during synaptogenesis, and Smaug1 knockdown affected the number and size of synapses, and also provoked an impaired response to repetitive depolarizing stimuli, as indicated by a reduced induction of Arc/Arg3.1. Our results suggest that S-foci control local translation, specifically responding to NMDA receptor stimulation and affecting synaptic plasticity. © 2011 Baez et al. |
| author |
Baez, María Verónica Luchelli, Luciana Maschi, Darío Habif, Martín Thomas, María Gabriela Boccaccio, Graciela Lidia |
| author_facet |
Baez, María Verónica Luchelli, Luciana Maschi, Darío Habif, Martín Thomas, María Gabriela Boccaccio, Graciela Lidia |
| author_sort |
Baez, María Verónica |
| title |
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| title_short |
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| title_full |
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| title_fullStr |
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| title_full_unstemmed |
Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation |
| title_sort |
smaug1 mrna-silencing foci respond to nmda and modulate synapse formation |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00219525_v195_n7_p1141_Baez http://hdl.handle.net/20.500.12110/paper_00219525_v195_n7_p1141_Baez |
| work_keys_str_mv |
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| _version_ |
1768544441327419392 |