Liver ferrochelatase from normal and hexachlorobenzene porphyric rats. mechanism of drug action

1. 1. The action of hexachlorobenzene (HCB) on hepatic ferrochelatase was investigated. 2. 2. A direct action of HCB, pentachlorophenol, porphyrins and haem on this enzyme activity was discarded. 3. 3. In HCB porphyric liver there is probably an activator tightly bound to the enzyme. 4. 4. Pyridoxal...

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Guardado en:
Detalles Bibliográficos
Publicado: 1991
Materias:
ferrochelatase
hexachlorobenzene
pyridoxal 5 phosphate
animal experiment
animal model
animal tissue
article
conformational transition
drug mechanism
drug toxicity
liver
nonhuman
oral drug administration
porphyria
rat
Animal
Binding Sites
Chromatography, Gel
Copper
Copper Sulfate
Female
Ferrochelatase
Heat
Heme
Hexachlorobenzene
Liver
Pentachlorophenol
Porphyria
Porphyrins
Protein Conformation
Pyridoxal Phosphate
Rats
Rats, Inbred Strains
Spectrophotometry
Support, Non-U.S. Gov't
Animalia
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0020711X_v23_n7-8_p669_RiosDeMolina
http://hdl.handle.net/20.500.12110/paper_0020711X_v23_n7-8_p669_RiosDeMolina
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. The action of hexachlorobenzene (HCB) on hepatic ferrochelatase was investigated. 2. 2. A direct action of HCB, pentachlorophenol, porphyrins and haem on this enzyme activity was discarded. 3. 3. In HCB porphyric liver there is probably an activator tightly bound to the enzyme. 4. 4. Pyridoxal phosphate (PPL) may be a cofactor of ferrochelatase from both normal and porphyric rats. 5. 5. The PPL would be involved in the binding site of Fe2+ or at least in the approaching of Fe2+ to the active site of the enzyme. 6. 6. The differences found between normal and porphyric preparations could be attributed to conformational changes elicited by the HCB. © 1991.

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