Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars

The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solu...

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Publicado:	1974
Materias:	lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v43_n1_p93_Garcia http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v43_n1_p93_Garcia
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spelling	paper:paper_00142956_v43_n1_p93_Garcia2025-07-30T17:19:29Z Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved 1974 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v43_n1_p93_Garcia http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars
spellingShingle	lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
topic_facet	lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars
description	The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved
title	Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
title_short	Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
title_full	Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
title_fullStr	Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
title_full_unstemmed	Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
title_sort	polysaccharide biosynthesis in acetobacter xylinum enzymatic synthesis of lipid diphosphate and monophospate sugars
publishDate	1974
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v43_n1_p93_Garcia http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia
_version_	1840322930299371520

Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars

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