Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoi...
Guardado en:
| Autores principales: | , |
|---|---|
| Publicado: |
1989
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v181_n2_p531_CACERES http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES |
| Aporte de: |
| Sumario: | Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved |
|---|