Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Publicado: |
1992
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v131_n3_p1223_Bley http://hdl.handle.net/20.500.12110/paper_00137227_v131_n3_p1223_Bley |
| Aporte de: |
| Sumario: | Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations were amplified by methylisobutylxan- thine an inhibitor of phosphodiesterase activity. The effect of both peptides were also enhanced by low doses of (Bu)2cAMP (0.2-1 mM). In contrast, higher concentrations were inhibitory. Similarly, FSH produced a biphasic enhancement of the insulin- and IGF-I-stimulated DNA synthesis. Maximal effects (2- to 6-fold increases) were observed with the lower doses (2-20 ng/ml) of the gonadotropin. FSH enhancement of IGF-I-stimulated DNA synthesis was dependent on cell density. Plating densities of 3-5 X 105 cells/cm2 were required for a maximal interaction. It is concluded that FSH, acting through a cAMP-mediated pathway, may regulate granulosa cell proliferation by modulating the mitogenic effects of insulin and/or IGF-I. © 1992 by The Endocrine Society. |
|---|