Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients

Background/Aims: The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical sme...

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Autores principales: Farías, Adrián Alejandro, Re, Viviana Elizabeth, Mengarelli, Silvia, Kremer, Luis, Pisano, María Belén, Allende, Luis, Nicolas, Juan Carlos, Elbarcha, Osvaldo César, Contigiani, Marta Silvia
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2010
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4998/1/A_Farias.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4998
record_format dspace
spelling I38-R144-49982025-11-06T16:24:15Z http://pa.bibdigital.ucc.edu.ar/4998/ Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients Farías, Adrián Alejandro Re, Viviana Elizabeth Mengarelli, Silvia Kremer, Luis Pisano, María Belén Allende, Luis Nicolas, Juan Carlos Elbarcha, Osvaldo César Contigiani, Marta Silvia R Medicina (General) Background/Aims: The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. Methodology: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. Results: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV/HIV coinfection, and epidemiological host factors. Conclusions: Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load and HCV/HIV coinfection. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV. 2010-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4998/1/A_Farias.pdf Farías, Adrián Alejandro, Re, Viviana Elizabeth ORCID: https://orcid.org/0000-0002-4106-6642 <https://orcid.org/0000-0002-4106-6642>, Mengarelli, Silvia, Kremer, Luis, Pisano, María Belén, Allende, Luis, Nicolas, Juan Carlos ORCID: https://orcid.org/0000-0001-9163-2668 <https://orcid.org/0000-0001-9163-2668>, Elbarcha, Osvaldo César and Contigiani, Marta Silvia ORCID: https://orcid.org/0000-0002-7171-0013 <https://orcid.org/0000-0002-7171-0013> (2010) Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients. Hepato-Gastroenterology, 57 (98). pp. 300-304. ISSN 01726390
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Farías, Adrián Alejandro
Re, Viviana Elizabeth
Mengarelli, Silvia
Kremer, Luis
Pisano, María Belén
Allende, Luis
Nicolas, Juan Carlos
Elbarcha, Osvaldo César
Contigiani, Marta Silvia
Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
topic_facet R Medicina (General)
description Background/Aims: The possibility of the non-parenteral Hepatitis C Virus (HCV) transmission is supported by the demonstration that the actual virus is present in several body fluids. In this study, we investigated the relationship between the detection of HCV RNA in body fluids (saliva, cervical smears, seminal fluid and peripheral blood mononuclear cells) from chronically HCV-infected patients and several viral and host factors. Methodology: This study comprised 16 HIV/HCV coinfected and 21 HCV monoinfected patients with a median age of 38 and 45 years, respectively. HCV-RNA was detected in serum and fluids samples by reverse transcription-nested polymerase chain reaction. Genotypes were determined by using RFLP and direct nucleotide sequencing of the PCR products and plasma viral loads by using NASBA HCV-QT. Results: When compared on the basis of the results of the detection of HCV-RNA in fluids, patients did not differ significantly in relation to viral load, genotype, HCV/HIV coinfection, and epidemiological host factors. Conclusions: Our data suggest that HCV can be detected in body fluids of chronically HCV-infected patients independent of these cofactors, including circulating HCV load and HCV/HIV coinfection. Studies on HCV dynamics are needed to gain insights into nonparenteral transmission of HCV.
format Artículo
PeerReviewed
author Farías, Adrián Alejandro
Re, Viviana Elizabeth
Mengarelli, Silvia
Kremer, Luis
Pisano, María Belén
Allende, Luis
Nicolas, Juan Carlos
Elbarcha, Osvaldo César
Contigiani, Marta Silvia
author_facet Farías, Adrián Alejandro
Re, Viviana Elizabeth
Mengarelli, Silvia
Kremer, Luis
Pisano, María Belén
Allende, Luis
Nicolas, Juan Carlos
Elbarcha, Osvaldo César
Contigiani, Marta Silvia
author_sort Farías, Adrián Alejandro
title Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_short Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_full Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_fullStr Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_full_unstemmed Detection of hepatitis C virus (HCV) in body fluids from HCV monoinfected and HCV/HIV coinfected patients
title_sort detection of hepatitis c virus (hcv) in body fluids from hcv monoinfected and hcv/hiv coinfected patients
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/4998/1/A_Farias.pdf
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