Disruption of antigenic variation is crucial for effective parasite vaccine

Giardia lamblia is a human intestinal pathogen. Like many protozoan microorganisms, Giardia undergoes antigenic variation, a mechanism assumed to allow parasites to evade the host's immune response, producing chronic and/or recurrent infections. Recently, we found that the mechanism controlling...

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Autores principales: Rivero, Fernando David, Saura, Alicia, Prucca, César Germán, Carranza, Pedro Gabriel, Torri, Alessandro, Luján, Hugo Daniel
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2010
Materias:
R Medicina (General)
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4987/1/A_Rivero.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4987
record_format dspace
spelling I38-R144-49872025-11-05T13:54:58Z http://pa.bibdigital.ucc.edu.ar/4987/ Disruption of antigenic variation is crucial for effective parasite vaccine Rivero, Fernando David Saura, Alicia Prucca, César Germán Carranza, Pedro Gabriel Torri, Alessandro Luján, Hugo Daniel R Medicina (General) Giardia lamblia is a human intestinal pathogen. Like many protozoan microorganisms, Giardia undergoes antigenic variation, a mechanism assumed to allow parasites to evade the host's immune response, producing chronic and/or recurrent infections. Recently, we found that the mechanism controlling variant-specific surface protein (VSP) switching in Giardia involves components of the RNA interference machinery and that disruption of this pathway generates trophozoites simultaneously expressing many VSPs. Here we use these altered trophozoites to determine the role of antigenic variation in a gerbil model of giardiasis. Our results show that either primary infection with trophozoites simultaneously expressing many VSPs or immunization with purified VSPs from the transgenic cells protects gerbils from subsequent Giardia infections. These results constitute, to our knowledge, the first experimental evidence that antigenic variation is essential for parasite survival within hosts and that artificial disruption of this mechanism might be useful in generating vaccines against major pathogens that show similar behavior. 2010-12-31 Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4987/1/A_Rivero.pdf Rivero, Fernando David, Saura, Alicia ORCID: https://orcid.org/0000-0003-1537-506X <https://orcid.org/0000-0003-1537-506X>, Prucca, César Germán ORCID: https://orcid.org/0000-0003-4165-4660 <https://orcid.org/0000-0003-4165-4660>, Carranza, Pedro Gabriel ORCID: https://orcid.org/0000-0002-1145-3852 <https://orcid.org/0000-0002-1145-3852>, Torri, Alessandro and Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315> (2010) Disruption of antigenic variation is crucial for effective parasite vaccine. Nature Medicine, 16 (5). pp. 551-557. ISSN 1546170X info:eu-repo/semantics/altIdentifier/doi/10.1038/nm.2141
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic R Medicina (General)
spellingShingle R Medicina (General)
Rivero, Fernando David
Saura, Alicia
Prucca, César Germán
Carranza, Pedro Gabriel
Torri, Alessandro
Luján, Hugo Daniel
Disruption of antigenic variation is crucial for effective parasite vaccine
topic_facet R Medicina (General)
description Giardia lamblia is a human intestinal pathogen. Like many protozoan microorganisms, Giardia undergoes antigenic variation, a mechanism assumed to allow parasites to evade the host's immune response, producing chronic and/or recurrent infections. Recently, we found that the mechanism controlling variant-specific surface protein (VSP) switching in Giardia involves components of the RNA interference machinery and that disruption of this pathway generates trophozoites simultaneously expressing many VSPs. Here we use these altered trophozoites to determine the role of antigenic variation in a gerbil model of giardiasis. Our results show that either primary infection with trophozoites simultaneously expressing many VSPs or immunization with purified VSPs from the transgenic cells protects gerbils from subsequent Giardia infections. These results constitute, to our knowledge, the first experimental evidence that antigenic variation is essential for parasite survival within hosts and that artificial disruption of this mechanism might be useful in generating vaccines against major pathogens that show similar behavior.
format Artículo
PeerReviewed
author Rivero, Fernando David
Saura, Alicia
Prucca, César Germán
Carranza, Pedro Gabriel
Torri, Alessandro
Luján, Hugo Daniel
author_facet Rivero, Fernando David
Saura, Alicia
Prucca, César Germán
Carranza, Pedro Gabriel
Torri, Alessandro
Luján, Hugo Daniel
author_sort Rivero, Fernando David
title Disruption of antigenic variation is crucial for effective parasite vaccine
title_short Disruption of antigenic variation is crucial for effective parasite vaccine
title_full Disruption of antigenic variation is crucial for effective parasite vaccine
title_fullStr Disruption of antigenic variation is crucial for effective parasite vaccine
title_full_unstemmed Disruption of antigenic variation is crucial for effective parasite vaccine
title_sort disruption of antigenic variation is crucial for effective parasite vaccine
publishDate 2010
url http://pa.bibdigital.ucc.edu.ar/4987/1/A_Rivero.pdf
work_keys_str_mv AT riverofernandodavid disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
AT sauraalicia disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
AT pruccacesargerman disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
AT carranzapedrogabriel disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
AT torrialessandro disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
AT lujanhugodaniel disruptionofantigenicvariationiscrucialforeffectiveparasitevaccine
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