Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves

Production of probiotic products requires preliminary research studies on technological characteristics of strains to verify the growth capacity of microorganisms and their survival rate and stability during storage. On an industrial scale production, it is important to lower the manufacturing cost...

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Autores principales: Soto, L. P., Frizzo, Laureano S., Bertozzi, E., Díaz, A., Marti, L.E., Santma, R. Dalla, Sequeira, Gabriel Jorge, Rosmini, Marcelo
Formato: Artículo PeerReviewed
Lenguaje:Español
Publicado: 2009
Materias:
SB Cultura de la planta
SF Cultura de los animales
Acceso en línea:http://pa.bibdigital.ucc.edu.ar/4929/1/A_Soto.pdf
Aporte de:
Producción Académica Universidad Católica de Córdoba (UCCor) de Universidad Católica de Córdoba
id I38-R144-4929
record_format dspace
spelling I38-R144-49292025-10-07T17:42:59Z http://pa.bibdigital.ucc.edu.ar/4929/ Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves Soto, L. P. Frizzo, Laureano S. Bertozzi, E. Díaz, A. Marti, L.E. Santma, R. Dalla Sequeira, Gabriel Jorge Rosmini, Marcelo SB Cultura de la planta SF Cultura de los animales Production of probiotic products requires preliminary research studies on technological characteristics of strains to verify the growth capacity of microorganisms and their survival rate and stability during storage. On an industrial scale production, it is important to lower the manufacturing cost by using inexpensive media both for propagation and preservation. The objective of the present study was to evaluate milk medium capacity to act as propagation matrix and cold preservation for a potential probiotic bacterial inoculum to be used in young calves bred in intensive systems. Bovine origin strains under study were Lactobacillus casei DSPV 318T, Lactobacillus salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T. Strain propagation and preservation capacities were evaluated in 100 g L-1 skim milk powder and de Man, Rogosa and Sharpe medium (MRS) as positive control. Cultures were stored at refrigerated (4°C) and freezing (-20°C) conditions and their viabilities rates were recorded every 21 days for a 3-months period and on months 6, 9 and 12 (180, 270 and 360 days). Biomass production and counts of cell viabilities were done in decimal dilutions in Ringer 1/4 solution and spread in MRS agar dishes. The 3-studied Lactic Acid Bacteria (LAB) were able to propagate in milk medium. This inoculum preservation, with bacterial counts higher than the Suggested Minimum Level (SML) (106 CFU Ml-1) in milk medium, was feasible when it was stored under refrigerated conditions for 84 days and in freezing conditions for 360 days. Manufacturing cost could be substantially reduced if the same medium could be successively used in both, propagation and conservation processes, modifying only the physical conditions. 2009-12-31 info:eu-repo/semantics/closedAccess Artículo PeerReviewed application/pdf spa http://pa.bibdigital.ucc.edu.ar/4929/1/A_Soto.pdf Soto, L. P., Frizzo, Laureano S. ORCID: https://orcid.org/0000-0002-4354-4564 <https://orcid.org/0000-0002-4354-4564>, Bertozzi, E., Díaz, A., Marti, L.E., Santma, R. Dalla, Sequeira, Gabriel Jorge ORCID: https://orcid.org/0000-0003-1332-7988 <https://orcid.org/0000-0003-1332-7988> and Rosmini, Marcelo ORCID: https://orcid.org/0000-0002-4171-411X <https://orcid.org/0000-0002-4171-411X> (2009) Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves. Journal of Animal and Veterinary Advances, 8 (7). pp. 1353-1360. ISSN 1993601X
institution Universidad Católica de Córdoba
institution_str I-38
repository_str R-144
collection Producción Académica Universidad Católica de Córdoba (UCCor)
language Español
orig_language_str_mv spa
topic SB Cultura de la planta
SF Cultura de los animales
spellingShingle SB Cultura de la planta
SF Cultura de los animales
Soto, L. P.
Frizzo, Laureano S.
Bertozzi, E.
Díaz, A.
Marti, L.E.
Santma, R. Dalla
Sequeira, Gabriel Jorge
Rosmini, Marcelo
Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
topic_facet SB Cultura de la planta
SF Cultura de los animales
description Production of probiotic products requires preliminary research studies on technological characteristics of strains to verify the growth capacity of microorganisms and their survival rate and stability during storage. On an industrial scale production, it is important to lower the manufacturing cost by using inexpensive media both for propagation and preservation. The objective of the present study was to evaluate milk medium capacity to act as propagation matrix and cold preservation for a potential probiotic bacterial inoculum to be used in young calves bred in intensive systems. Bovine origin strains under study were Lactobacillus casei DSPV 318T, Lactobacillus salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T. Strain propagation and preservation capacities were evaluated in 100 g L-1 skim milk powder and de Man, Rogosa and Sharpe medium (MRS) as positive control. Cultures were stored at refrigerated (4°C) and freezing (-20°C) conditions and their viabilities rates were recorded every 21 days for a 3-months period and on months 6, 9 and 12 (180, 270 and 360 days). Biomass production and counts of cell viabilities were done in decimal dilutions in Ringer 1/4 solution and spread in MRS agar dishes. The 3-studied Lactic Acid Bacteria (LAB) were able to propagate in milk medium. This inoculum preservation, with bacterial counts higher than the Suggested Minimum Level (SML) (106 CFU Ml-1) in milk medium, was feasible when it was stored under refrigerated conditions for 84 days and in freezing conditions for 360 days. Manufacturing cost could be substantially reduced if the same medium could be successively used in both, propagation and conservation processes, modifying only the physical conditions.
format Artículo
PeerReviewed
author Soto, L. P.
Frizzo, Laureano S.
Bertozzi, E.
Díaz, A.
Marti, L.E.
Santma, R. Dalla
Sequeira, Gabriel Jorge
Rosmini, Marcelo
author_facet Soto, L. P.
Frizzo, Laureano S.
Bertozzi, E.
Díaz, A.
Marti, L.E.
Santma, R. Dalla
Sequeira, Gabriel Jorge
Rosmini, Marcelo
author_sort Soto, L. P.
title Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
title_short Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
title_full Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
title_fullStr Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
title_full_unstemmed Milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
title_sort milk evaluation as growth and cold preservation medium of a probiotic inoculum for young calves
publishDate 2009
url http://pa.bibdigital.ucc.edu.ar/4929/1/A_Soto.pdf
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