Transport of charged small molecules after electropermeabilization — drift and diffusion

"We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO...

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Detalles Bibliográficos
Autores principales: Sözer, Esin B., Pocetti, Florencia, Vernier, P. Thomas
Formato: Artículos de Publicaciones Periódicas publishedVersion
Lenguaje:Inglés
Publicado: 2019
Materias:
MEMBRANAS
MOLECULAS
TRANSPORTE
CELULAS
Acceso en línea:http://ri.itba.edu.ar/handle/123456789/1582
Aporte de:
Repositorio Institucional Instituto Tecnológico de Buenos Aires (ITBA) de Instituto Tecnológico de Buenos Aires (ITBA)
Descripción
Sumario:"We provide a direct quantitative comparison of molecular transport of three similarly sized but chemically different fluorescent indicators of membrane permeabilization — two cationic molecules (YO-PRO-1 and propidium), and one anionic molecule (calcein). Our results show that the influx of YO-PRO-1 and propidium (both cations) into electropermeabilized cells is an order of magnitude greater than that of calcein (an anion) after the same pulse exposure. Calcein efflux from cells loaded before permeabilization, however, is similar in magnitude to YO-PRO-1 and propidium influx."

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