Thiol groups of soybean callus succinyl CoA synthetase

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1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of...

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Autores principales:	De Xifra, E.A.W., Del C. Batlle, A.M.
Formato:	Artículo publishedVersion
Publicado:	1974
Materias:	5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_0020711X_v5_n2_p129_DeXifra_oai
Aporte de:	Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires

id	I28-R145-paper_0020711X_v5_n2_p129_DeXifra_oai
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spelling	I28-R145-paper_0020711X_v5_n2_p129_DeXifra_oai2024-08-16 De Xifra, E.A.W. Del C. Batlle, A.M. 1974 1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. application/pdf http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar Int. J. Biochem. 1974;5(2):129-135 5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups Thiol groups of soybean callus succinyl CoA synthetase info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_0020711X_v5_n2_p129_DeXifra_oai
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-145
collection	Repositorio Digital de la Universidad de Buenos Aires (UBA)
topic	5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups
spellingShingle	5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups De Xifra, E.A.W. Del C. Batlle, A.M. Thiol groups of soybean callus succinyl CoA synthetase
topic_facet	5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups
description	1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974.
format	Artículo Artículo publishedVersion
author	De Xifra, E.A.W. Del C. Batlle, A.M.
author_facet	De Xifra, E.A.W. Del C. Batlle, A.M.
author_sort	De Xifra, E.A.W.
title	Thiol groups of soybean callus succinyl CoA synthetase
title_short	Thiol groups of soybean callus succinyl CoA synthetase
title_full	Thiol groups of soybean callus succinyl CoA synthetase
title_fullStr	Thiol groups of soybean callus succinyl CoA synthetase
title_full_unstemmed	Thiol groups of soybean callus succinyl CoA synthetase
title_sort	thiol groups of soybean callus succinyl coa synthetase
publishDate	1974
url	http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra https://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_0020711X_v5_n2_p129_DeXifra_oai
work_keys_str_mv	AT dexifraeaw thiolgroupsofsoybeancallussuccinylcoasynthetase AT delcbatlleam thiolgroupsofsoybeancallussuccinylcoasynthetase
_version_	1809356775105757184

Thiol groups of soybean callus succinyl CoA synthetase

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