Haplotipos mitocondriales fundadores en Colonias Unidas, departamento de Itapúa, Paraguay
Between the end of the XIX century and the beginning of the XX, thousands of German families migrated from their homes, seeing departure the only alternative for growth and hope for greater well-being after wars, famine, political, religious, and economic crises in their native country. Thus, a grou...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Tesis de maestría acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2023
|
| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_7956 https://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_7956.dir/7956.PDF |
| Aporte de: |
| Sumario: | Between the end of the XIX century and the beginning of the XX, thousands of German families migrated from their homes, seeing departure the only alternative for growth and hope for greater well-being after wars, famine, political, religious, and economic crises in their native country. Thus, a group of German immigrants saw the Republic of Paraguay as an attractive place to start a new life. In this way, they colonized the current localities of Hohenau, later Obligado and finally Bella Vista, three independent municipalities which are nowadays recognized as "Colonias Unidas" (United Colonies). Considering the origin of the population of these districts in the department of Itapúa, Paraguay, the aim of the present work was to contribute to the characterization of part of the Paraguayan population through the analysis of a genetic marker of uniparental inheritance. Specifically, the hypervariable segments of the mitochondrial DNA control region identified as HVS- I and HVS- II were sequenced. The population analysed was represented by 75 samples of unrelated individuals from the localities of Hohenau (N= 28), Obligado (N= 28) and Bella Vista (N= 19). From the sequences obtained, haplotypic diversity, nucleotide diversity, interhaplotype genetic distances were calculated, the relationship between mitochondrial haplotypes was obtained through the phylogenetic network and each haplotype was assigned to the haplogroup to which it belongs according to the EMPOP database. A total of 60 mitochondrial haplotypes were identified and showed a high haplotypic diversity in each locality corresponding to 0.997 (± 0.010); 0.989 (± 0.019) and 0.994 (± 0.010) for Hohenau, Obligado, Bella Vista respectively. For Colonias Unidas as a whole, the haplotypic diversity was Hd= 0.992 (± 0.004) and the nucleotide diversity was Pi= 0.00992. Likewise, although a high genetic diversity was observed, the genetic distance among the groups of samples belonging to the three localities studied was low (? 0.01559), reflecting what is socially proclaimed, Colonias Unidas self-perceived itself as a unit. The haplotypic diversity was finally grouped into 14 haplogroups A, C, D, H, I, J, JT, K, L, N, R, T, U and W, identifying a high proportion of haplotypes (80%) corresponding to haplogroups H, I, J, JT, K, R, T, U and W registered as European, highlighting a matrilineal ancestry of European origin and showing haplogroup H as the founder of the Colonias Unidas population with a frequency of 0.33. The remaining 17% of the haplogroups corresponded to Native American ancestry in contrast to that informed by other authors (87.2%) and 3% to African and Middle Eastern ancestry, similar to that reported in the literature for other Paraguayan populations (4%, 5% respectively). The identification of the U haplotype not mentioned for Paraguay in the literature also noteworthy. Thus, the data obtained in the present study suggest that in some Paraguayan populations the miscegenation reported between Amerindian women and European men is not observed, given the low proportion of Amerindian haplotypes observed in Colonias Unidas. This could be due to cultural issues that remain entrenched in this district of Paraguay. |
|---|