Mejoramiento genético de lechuga a través del knock out del gen LsSPL13 vía CRISPR/Cas9

The transcription factor SPL13 is related to vegetative growth and development. Its silencing in alfalfa produces plants with a longer vegetative phase and an increase in leaf area, characteristics of interest for lettuce production. The objective of this thesis was to implement gene editing via CRI...

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Detalles Bibliográficos
Autor principal: Beracochea, Valeria Cecilia
Otros Autores: López Bilbao, Marisa
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica 2024
Materias:
Lechuga
Edición génica
CRISPR/Cas9
Knock out
SPL13
Biomasa
Floración
Lettuce
Gene editing
Biomass
Flowering.
Ciencias de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_7846
https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_7846.dir/7846.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The transcription factor SPL13 is related to vegetative growth and development. Its silencing in alfalfa produces plants with a longer vegetative phase and an increase in leaf area, characteristics of interest for lettuce production. The objective of this thesis was to implement gene editing via CRISPR/Cas9 in lettuce (Lactuca sativa), through the knock out of the LsSPL13 gene and to obtain plants with increased biomass and delayed flowering. During this thesis, the lettuce SPL13 gene was identified, 3 guide RNAs were designed and the editing vector was assembled and introduced via Agrobacterium tumefaciens into lettuce. The 20 transgenic events obtained were molecularly characterized, resulting in 3 edited lines. Phenotypic analysis of E1 and E2 lines, up to the T3 generation, showed in comparison with the control plants: increased biomass, greater number of leaves, delayed flowering, larger seeds and greater number of secondary roots, while no differences were observed in the centesimal composition analysis. Furthermore, E1 would be an edited non-transgenic line. In this way, the stated objectives were achieved and the biotechnological interest of the SPL13 gene was demonstrated.

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