InVet 2021, 23 (1): 01-9 ARTÍCULO DE INVESTIGACIÓN ISSN...

Campylobacter fetus is a Gram negative bacterium, with 2 relevant subspecies for cattle health: C. fetus\nsubsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv) which cause important reproductive losses. In the\npresent work, four groups of bulls were used: one group to characterize the genital mic...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: García, J.A., Soto, J., Soto, P., Malena, R., Morsella, C., Méndez, A., Fiorentino, M.A., Acuña, J., Lucchesi, E., Paolicchi, F.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: Universidad de Buenos Aires. Facultad de Ciencias Veterinarias 2021
Materias:
campilobacteriosis genital bovina
rumiantes
enfermedad venérea
abortos
infertilidad
bovine genital campylobacteriosis
ruminants
venereal disease
abortions
infertility
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_6756
https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_6756.dir/6756.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Campylobacter fetus is a Gram negative bacterium, with 2 relevant subspecies for cattle health: C. fetus\nsubsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv) which cause important reproductive losses. In the\npresent work, four groups of bulls were used: one group to characterize the genital microbiota (G1; n=3)\nand the other three to evaluate the direct immunofluorescence (DIF) test for the diagnosis of bovine\ncampylobacteriosis in preputial samples (PS) from experimentally infected bulls with Cff (G2; n=3) and Cfv (G3; n=3), and controls (G4; n=4). Seven bacterial strains of different genera were identified in the PS from G1. Other 11 laboratory bacterial strains were included: Cff, Cfv and Cfv biovar Intermedius, 3 Campylobacter non-fetus, 3 Gram negative and 2 Gram positive. Only the C. fetus strains presented positive immunostaining. The sensitivity and specificity of the DIF test were 79 % and 100 %, respectively. Concordance between DIF test and bacteriological culture was 83 % (Kappa index: 0.65). The present study shows the high specificity of the DIF test for detection of C. fetus using a fluorescent conjugate elaborated in Argentina, and highlights the relevance of no cross reaction against 15 bacterial strains normally present in bull preputial microbiota.

Ejemplares similares