Análisis de la compatibilidad de los segmentos internos del genoma de aislamientos argentinos de virus de Influenza A
Influenza A isolates from wild bird in Argentina belong to a new and unique lineage. Furthermore, there has not been identified internal genes belonging to a different lineage in this region. Besides, infectivity of these viruses has shown to be limited in chicken. In this work we evaluated: (i) Ad...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2016
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6412 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6412.dir/6412.PDF |
| Aporte de: |
| Sumario: | Influenza A isolates from wild bird in Argentina belong to a new and unique lineage. Furthermore, there has not been identified internal genes belonging to a different lineage in this region. Besides, infectivity of these viruses has shown to be limited in chicken. In this work we evaluated: (i) Adaptability of an H4N2 Argentinian isolate through serial passages in chicken and (ii) its reassortment capability perfoming coinfections with viruses from different latitudes. \nAfter five passages in chicken virus was detected until day four posinfection in contrast to its wild type that was not detected after day two posinfection. After sequencing its genome aminoacids substitutions involved in host switch were elucidated.\nIn vitro coinfection using a virus carrying internal genes from Asia showed limited reassortment. On the other hand, coinfection with a virus carrying internal genes from Northamerica showed a higher percentage of reassortants and more diverse constellations. Lung explants infections showed that the most frequent reassortants gained that ability to infect this tissue. This work shows for the first time that an Argentinian isolate was able to improve its infectivity in chicken model and that was able to reassort its internal genes with viruses from other latitudes. |
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