Diseño de protocolos imagenológicos y nano sondas radiomarcadas con 99mTc. Evaluación de su potencial uso en el área de oncología preclínica para el monitoreo de intervenciones terapéuticas y diagnósticas
Development of imaging protocols and radioactive nano probes with 99mTc. Evaluation of their potential use in preclinical oncology for the follow up of therapeutic and diagnostic interventions\nThe present work shows the development of: i- imaging gamma camera protocols that allow to evidence delete...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6388 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6388.dir/6388.PDF |
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| Sumario: | Development of imaging protocols and radioactive nano probes with 99mTc. Evaluation of their potential use in preclinical oncology for the follow up of therapeutic and diagnostic interventions\nThe present work shows the development of: i- imaging gamma camera protocols that allow to evidence deletereal effects of doxorubicin in cardiac, hepatic and renal systems in animal models; ii- radioactive probes with diagnostic use in breast cancer evaluated in animal models by means of gamma camera imaging. Results demostrated the utility of this gamma camera imaging protocols to show doxorubicin adverse effects in heart, liver and kidneys for rats and just kidneys for mice. Imagenological mathematical parameters were calculated: heart/background (99mTc-sestamibi), %liver/bone marrow (99mTc-fitato), %IDkidneys (99mTc-DMSA). These results represent a new and valuable strategy that will be of much use to continue doxorubicin research, in particular, though it will also allow safety and efficacy evaluation of other therapeutic and chemoprotective drugs, respectively.\nThe in vivo performance of the nano micellar systems (TPGS; TPGS and Soluplus®; TPGS and Soluplus® with glucose) is also shown, regarding their potencial use as diagnostic imaging probes, which showed that only the mixed micellar system (TPGS and Soluplus®) allowed the visualization of the tumor in the imaging assays for the singeneic model of 4T1 cells. TPGS nano micelles resulted in a better diagnostic value than comercial 99mTc-sestamibi for the chemically induced model with NMU. These represent the ground results for the development of a SPECT diagnostic probe for breast cancer, compatible with radiopharmaceutical practice in nuclear medicine services in Argentina. |
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