Superantígenos del operón egc. Efectos sobre células de la inmunidad innata
Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR molecules, activating as much as 20 % of the T cell population and promoting a cytokine storm which drives to endotoxic shock, causing immunosuppression and hindering the immune response against bacterial infection. Since mo...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2020
|
| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6378 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6378.dir/6378.PDF |
| Aporte de: |
| Sumario: | Bacterial superantigens (SAgs) are enterotoxins that bind to MHC-II and TCR molecules, activating as much as 20 % of the T cell population and promoting a cytokine storm which drives to endotoxic shock, causing immunosuppression and hindering the immune response against bacterial infection. Since monocytes/macrophages, neutrophils and ?? T cells are the firsts cells SAgs find in infected host and considering the effect of these cells have on directing the immune response, here, we investigated the effect of four non-classical SAgs encoded in the staphylococcal egc operon, namely, SEG, SEI, SEM and SEO on these subsets of innate immune cells. We found that egc SAgs depleted the pool of innate immune effector cells inducing an inefficient activation of monocytic/macrophagic cells, at the same time that induced activation as well as death of neutrophils and ?? T cells, driving the immune response to an impaired proinflammatory profile. Additionally, we proved that part of the effects is mediated directly or indirectly by interactions with MHC-II. In addition, performing surface plasmon resonance assays (SPR), we demonstrated that non-classical SAgs bind the gp130 molecule, which is ubiquitously present in several populations of innate cells. |
|---|