Estudio del efecto del Virus de la Leucosis Bovina en el epitelio mamario humano y bovino
Bovine leukemia virus (BLV) is a retrovirus that infects cattle and is associated with an increase in secondary infections. The objective of this study was to infect in vitro a bovine and human mammary epithelial cell line (MAC-T and MCF10A, respectively) and analyze the effect of those infections....
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_6345 https://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_6345.dir/6345.PDF |
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| Sumario: | Bovine leukemia virus (BLV) is a retrovirus that infects cattle and is associated with an increase in secondary infections. The objective of this study was to infect in vitro a bovine and human mammary epithelial cell line (MAC-T and MCF10A, respectively) and analyze the effect of those infections. Our findings show that BLV stably infects MAC-T causing late syncytium formation, a decrease in cell viability, downregulation of the anti-apoptotic gene Bcl-2, and an increase in TLR9 and TNF-? mRNA expression. Moreover, in the presence of S. aureus, MAC-T BLV cells had decreased viability and decreased Bcl-2 and TLR2 mRNA expression. What is more, MAC-T BLV cells have altered expression of genes related to the innate immune response and defense against virus. All together, these results suggest that mammary epithelial cells infected with BLV have altered the apoptotic and immune pathways, probably affecting their response to bacteria and favoring the development of mastitis. On the other hand, MCF10A cells infected with BLV does not release viral proteins or ARN to the supernatant. Only a fragment of the viral gene pol was detected in the cellular DNA suggesting that the infection is not productive. |
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