Evaluación de la actividad biológica y protectora de la rúcula (Eruca sativa y Diplotaxis tenuifolia) en modelo in vivo
In vivo activity of two rocket species (Eruca sativa and Diplotaxis tenuifolia) was developed through effect biomarkers determination, in order to establish their possible adverse or beneficial effect, from a genetic toxicology point of view.\nThe rocket, acquired in an organic vegetable garden, was...
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| Formato: | Tesis de maestría acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=afamaster&cl=CL1&d=HWA_5920 http://repositoriouba.sisbi.uba.ar/gsdl/collect/afamaster/index/assoc/HWA_5920.dir/5920.PDF |
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| Sumario: | In vivo activity of two rocket species (Eruca sativa and Diplotaxis tenuifolia) was developed through effect biomarkers determination, in order to establish their possible adverse or beneficial effect, from a genetic toxicology point of view.\nThe rocket, acquired in an organic vegetable garden, was processed to obtain a juice. Three doses (1, 1.4 and 2 g / kg of body weight) were used to investigate genotoxic and antigenotoxic effects of these plants. Two widely recommended biomarkers were used to evaluate genotoxicity: micronucleus test in bone marrow and cell gel electrophoresis assay (comet assay) in peripheral blood.\nBoth methodologies confirmed that the juices of D. tenuifolia and E. vesicaria did not present a clastogenic or aneugenic behavior as well as cytotoxicity or DNA damage induction. In summary, they don?t have genotoxic effects.\nThe evaluation of the potential protective effect of both species was conducted using a drug that causes genetic damage in the animals used in this experimental design (cyclophosphamide), using a single dose administration. The studied biomarkers (Micronuclei and Comet assay) showed a statistically significant decrease in DNA damage, in relation to positive control. These results were directly proportional to the concentration of rocket used for both species. Therefore, we conclude that they have the capacity to attenuate damage induced by cyclophosphamide.\nThese findings suggests to D. Tenuifolia and E. Vesicaria as elements of diet that present promising characteristics to be used as a health promoting agent. |
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