Estudio del rol del sistema purinérgico en la regulación de la génesis celular y regeneración tisular en modelos experimentales de injuria retiniana en el zebrafish (Danio rerio) adulto
Zebrafish have the remarkable ability to regenerate injured tissues and organs. This trait makes them\nan excellent vertebrate model to study the processes involved to tissue repair. The extracellular\npurinergic system intervenes in the majority of the biological processes, including the embryonic\...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5801 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5801.dir/5801.PDF |
| Aporte de: |
| Sumario: | Zebrafish have the remarkable ability to regenerate injured tissues and organs. This trait makes them\nan excellent vertebrate model to study the processes involved to tissue repair. The extracellular\npurinergic system intervenes in the majority of the biological processes, including the embryonic\ndevelopment. In this thesis we investigated the involvement of the different components of the\npurinergic system during retina regeneration utilizing two different in vivo injury models with\nintravitreal injections. In one, we used a specific ouabain dose that affects all retina cells but primarily\nganglion cells and interneurons. The other consisted of a specific CoCl2 dose injection that injures\nalmost exclusively photoreceptor cells. The pharmacological manipulation of the different components\nof the purinergic system (enzymes, nucleotides, nucleosides and purinergic receptors), within the\nmicroenvironment of the injury, provoked drastic changes in proliferation and cell death, glial and\nmicroglial reactivity, angiogenesis and the transcriptional induction of a variety of genes involved in\nretina regeneration. By utilizing the knowledge on the regulatory mechanisms of the purinergic\nsystem and their role in tissue regeneration a specific set of therapies could develop in order to treat\nhuman angiogenic, neurodegenerative or proliferative retinopathies. |
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