Valoración de subproductos agroindustriales: obtención de enzimas de interés comercial a partir de cáscara de semillas de soja
Aspects related to the valorization of an agroindustrial byproduct (the soybean seed hull) were studied, by means of its use as a raw material to obtaining commercial interest enzymes. For this purpose, soybean seed hull extracts were obtained according to the optimized conditions, considering low c...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5793 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5793.dir/5793.PDF |
| Aporte de: |
| Sumario: | Aspects related to the valorization of an agroindustrial byproduct (the soybean seed hull) were studied, by means of its use as a raw material to obtaining commercial interest enzymes. For this purpose, soybean seed hull extracts were obtained according to the optimized conditions, considering low cost variables. After identifying the soybean seed hull extract proteins with a shotgun proteomic methodology, chitosan minispheres were obtained. Then, chitosan minispheres were conferred with mechanical strength by means of a chemical crosslinking. Furthermore, the chitosan minispheres were derivatized with different strategies, depending on protein?s amino acid structure. Then, matrices derivatization was carried out for protein purification applications in batch mode. These matrices were tested for the purification and/or immobilization of some identified soybean seed hull proteins: peroxidase, urease, trypsin inhibitors, chitinase and ?-amylase. |
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