Genómica comparativa y funcional aplicada a los mecanismos involucrados en el desarrollo hacia la extrema y pandroga resistencia antibiótica
We took the class 1 integrons as a biological model to study the mechanisms of the Horizontal Gene Transfer of Antimicrobial Resistance Genes. These genetic elements are associated with the Multidrug Resistance (MDR). The intI1 gene was maintained in clinical strains belonging to lineages with epide...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_5792 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_5792.dir/5792.PDF |
| Aporte de: |
| Sumario: | We took the class 1 integrons as a biological model to study the mechanisms of the Horizontal Gene Transfer of Antimicrobial Resistance Genes. These genetic elements are associated with the Multidrug Resistance (MDR). The intI1 gene was maintained in clinical strains belonging to lineages with epidemic behavior for more than 900 generations. Bioinformatics studies evidenced a dynamic flow of intI1 gene alleles in particular in human niches that have probably allowed their expansion since the appearance of humans due to vertical inheritance. When analyzing the adaptation of P. aeruginosa to the lung of a patient with Cystic Fibrosis during chronic colonization, we identified that mobile resistance acquisition events occurred simultaneously with genomic reduction. These events conferred a new genomic island named PApGI which has a class 1 integron carrying the gene cassette blaVIM-2. This gene is maintained over time in more than 1000 generations which makes genomic island PApGI an important reservoir that can potentially be transferred to other strains. These results demonstrate the adaptive role that class 1 integrons confer to diverse species and habitats, including chronic infections of P. aeruginosa. |
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