InVet. 2020, 22 (1): 41-49 ISSN 1514-6634 (impreso) USO DE LECITINA DE SOJA...
Egg yolk, used routinely in numerous semen freezing protocols, has the limitation of being a compound of animal origin and thus a potential risk of contamination. It must be processed at the time of its use and as it has granules similar in size to sperm, it interferes with the evaluation of semen....
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| Autores principales: | , , |
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| Formato: | Artículo publishedVersion |
| Lenguaje: | Español |
| Publicado: |
Universidad de Buenos Aires. Facultad de Ciencias Veterinarias.
2020
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=pveterinaria/invet&cl=CL1&d=HWA_3712 https://repositoriouba.sisbi.uba.ar/gsdl/collect/pveterinaria/invet/index/assoc/HWA_3712.dir/3712.PDF |
| Aporte de: |
| Sumario: | Egg yolk, used routinely in numerous semen freezing protocols, has the limitation of being a compound of animal origin and thus a potential risk of contamination. It must be processed at the time of its use and as it has granules similar in size to sperm, it interferes with the evaluation of semen. The objective of this study was to evaluate if AndroMed®, a medium based on soybean lecithin, can be used successfully to freeze equine semen. The ejaculates were diluted with: 1) egg yolk and dimethylformamide; 2) egg yolk, dimethylformamide and glycerol; 3) AndroMed®; 4) AndroMed® with dimethylformamide. The kinematic parameters, viability and acrosome status of post-thaw spermatozoa were evaluated. Total and progressive motility were significantly higher (p<0.05) in media with egg yolk when compared to AndroMed®. In addition, media with egg yolk showed significantly higher percentages of live sperm with intact acrosomes than those with AndroMed® (p<0.05). In conclusion, under the described experimental conditions, the AndroMed® diluent did not show any quantitative advantages over a conventional diluent with regard to its ability to freeze equine semen with minimal parameters of in vitro acceptability. |
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