Evaluación de las interacciones entre especies bacterianas promotoras del crecimiento vegetal y su incidencia en el desarrollo conjunto, para la generación de consorcios bacterianos
The response to the increasing demand for food should be given in a sustainability context. So, the use of bio-fertilizers is a overcoming practice, since it reduces the use of chemical fertilizer that negatively impact on the agro-ecosystems. The aim of this work was to develop a soy bean bio-ferti...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_3164 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_3164.dir/3164.PDF |
| Aporte de: |
| Sumario: | The response to the increasing demand for food should be given in a sustainability context. So, the use of bio-fertilizers is a overcoming practice, since it reduces the use of chemical fertilizer that negatively impact on the agro-ecosystems. The aim of this work was to develop a soy bean bio-fertilizer formed by three different bacterial genera. To achieve this objective, a biotechnological process was developed, in order to culture the microorganisms in the same fermentation broth. The interactions among microorganisms established by quorum sensing (QS) were assessed. All the assessed strains: Bradyrhizobium japonicum E109 (B), Azospirillum brasilense Az39 (A) y Pseudomonas fluorescens P3 (P) produced signaling molecules of the Acyl homoserine lactones (AHLs) kind. In B. japonicum, the presence of heterologous AHLs induced changes in the AHLs synthetized. In addition, the PGPR capacity of the strains was analyzed, being A. brasilense the main AIA producer and its production influenced by QS. On the other hand, P. fluorescens presented siderophores production and phosphorous solubilizing capacity, independently of QS. Finally, the best symbiotic performance of B. japonicus was achieved by the triple co-cultured consortium. This system represent a biotechnological alternative, where the bacterial PGPR synergically complement each other, achieving results that were not observed individually. |
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