Alteraciones neurológicas asociadas al Síndrome Urémico Hemolítico : compromiso inflamatorio en la encefalopatía producida por la toxina Shiga 2 y el lipopolisacárido
Shiga toxin 2 (Stx2) from Shiga toxin-producing Escherichia coli (STEC) produces hemorrhagic colitis, hemolytic uremic syndrome (HUS) and acute encephalopathy. The \nmortality rate in HUS increases significantly when the central nervous system (CNS) is involved. Besides Stx2, STEC also releases LPS....
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2019
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_3137 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_3137.dir/3137.PDF |
| Aporte de: |
| Sumario: | Shiga toxin 2 (Stx2) from Shiga toxin-producing Escherichia coli (STEC) produces hemorrhagic colitis, hemolytic uremic syndrome (HUS) and acute encephalopathy. The \nmortality rate in HUS increases significantly when the central nervous system (CNS) is involved. Besides Stx2, STEC also releases LPS. To establish the effects of Stx2, the contribution of LPS and the role of microglia in the encephalopathy, an in vivo (murine model) and in vitro (rat primary cell culture of microglia) model were employed. Stx2 \nproduced cognitive deficits, alters in the neurovascular unit of the hippocampal CA1 area, lipid peroxidation, released pro-inflammatory cytokines and activated of NF-kB signaling \npathway in an ERK 1/2 independent way. Moreover, this thesis described for the first time the expression of Gb3 (receptor of Stx2) on microglia, and when microglia is activated by \nthese toxins, it triggers and inflammatory response by releasing of cytokines and an increased phagocytic activity. All these results were aggravated by LPS. In conclusion, the \nwork that led to the development of this thesis determined that, Stx2 may damage the CNS directly, but it produced a more important damage indirectly as the toxins induced an \ninflammatory process with the microglial cell being the pivotal cells in the development of \nthis process. |
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