Participación de las acuaporinas y caveolina-1 en el desarrollo temprano de la placenta humana

Even though human placenta is a normal tissue; extravillous cytotrophoblast cells (CEV) share several attributes with tumor cells. Additionally, aquaporins (AQPs)and Cav-1 modulate cell migration and invasion during cancer development and are found altered on gestational disorders.\nHYPOTHESIS: AQPs...

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Detalles Bibliográficos
Autor principal: Reca, Alejandra
Otros Autores: Damiano, Alicia E.
Formato: Tesis doctoral acceptedVersion
Lenguaje:Español
Publicado: Facultad de Farmacia y Bioquímica 2019
Materias:
Citotrofoblasto
AQP
Cav-1
Aquaporinas
Caveolina
Placenta
Ciencias de la vida
Acceso en línea:http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2831
http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2831.dir/2831.PDF
Aporte de:
Repositorio Digital de la Universidad de Buenos Aires (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Even though human placenta is a normal tissue; extravillous cytotrophoblast cells (CEV) share several attributes with tumor cells. Additionally, aquaporins (AQPs)and Cav-1 modulate cell migration and invasion during cancer development and are found altered on gestational disorders.\nHYPOTHESIS: AQPs y Cav-1 regulate trophoblast migration and invasionduring early placental developmentand their altered expression/functionality leads to gestational disorders characterized by unsuccessful placentation.\nMATERIALS AND METHODS: Cell lines: Swan 71 (CEV) and EA.hy926 (endothelial). Cell migration and invasion assays: wound healing assay, transwell invasion assay and zymography. Angiogenesis assay: tube formation assay.\nRESULTS: siRNA-mediated AQP3 downregulation andcaveolae disruption via methyl-?-cyclodextrinreduced CEV migration. Cell invasion was only significantly abolished after inhibition of all AQP isoforms present, in accordance with MMP-2 activity reduction. Angiogenesis of CEV and endothelial cells was significantly increased after caveolae disruption.\nDISCUSSION: An altered AQP expression,specially of AQP3, and a reduced caveolae presence compromises early placental development and can lead to gestational disorders, such as preeclampsia and intrauterine growth restriction.

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