Rol de las Heat Shock Proteins en tejido renal de ratas Zucker Diabetic Fatty
The aim of this study was to evaluate whether the "Heat Shock Proteins" (HSP) can control functional and morphological renal damage in a recognized animal model of type 2 diabetes (DMT2). Male 12-week old "Zucker Diabetic Fatty" (ZDF) rats and control "Lean Zucker Rat¨ (LZR)...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2825 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2825.dir/2825.PDF |
| Aporte de: |
| Sumario: | The aim of this study was to evaluate whether the "Heat Shock Proteins" (HSP) can control functional and morphological renal damage in a recognized animal model of type 2 diabetes (DMT2). Male 12-week old "Zucker Diabetic Fatty" (ZDF) rats and control "Lean Zucker Rat¨ (LZR) were used. All animals received water and standard rat chow, "ad libitum". The immunoexpression of HSP70 and HSP27 was assessed in kidney tissue. Markers of glomerular and tubulointerstitial damage (oxidative stress, inflammation, nitrosative stress, fat deposition, apoptosis, interstitial fibrosis), and podocyte injury were analyzed. Ultrastructural glomerular alterations were evaluated by electron microscopy. We correlated biochemical variables and histological markers of podocyte damage and expression of HSPs in renal tissue. The conclusion of this study is that the increased expression of HSP70 and HSP27 found in kidney of ZDF rats, although it represents an activated repair mechanism in response to an adverse metabolic scenario, its participation does not succeed in modulating the injury caused in such tissue, showing insufficient or at least incomplete behavior due to the magnitude of renal cell damage induced in this experimental model of T2DM. |
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