Estrategias de rizorremediación de glifosato en suelos bajo explotación agrícola intensiva
The main objective of this thesis work is to generate the knowledge that allows developing a rhizoremediation system capable of mitigating the impact associated with the use of glyphosate in soils of the Argentine Humid Pampas under intensive agricultural exploitation. To achieve this aim, methodolo...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2815 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2815.dir/2815.PDF |
| Aporte de: |
| Sumario: | The main objective of this thesis work is to generate the knowledge that allows developing a rhizoremediation system capable of mitigating the impact associated with the use of glyphosate in soils of the Argentine Humid Pampas under intensive agricultural exploitation. To achieve this aim, methodologies were developed and implemented to evaluate root and foliar tolerance to glyphosate in plant species with productive value, together with phenotypic and genotypic isolation and characterization of tolerant and glyphosate-degrading bacterial strains, including the whole genome sequence of two of them. Finally, plant-microorganism interaction trials and a microcosm assay were carried out. Among the plant species evaluated, Lotus corniculatus was selected as a candidate for being able to tolerate an exposure to 5.0 mg Kg-1 of glyphosate through root and 700 g ha-1 through foliar application. A culture collection of glyphosate tolerant strains was obtained. The whole genome sequencing of Ochrobactrum sp. P6BS-III and Rhizobium sp. P44RR-XXI was performed. The microcosm assay revealed that L. corniculatus inoculated with Ochrobactrum sp. P6BS-III could result in an adequate rhizoremediation system. |
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