Evaluación de vacunas a subunidades recombinantes contra la colonización del bovino por Escherichia coli O157:H7, agente causal del Síndrome Urémico Hemolítico : análisis de parámetros inmunológicos
Escherichia coli O157:H7 is a zoonotic pathogen and the major etiologic agent of Haemolytic Uremic Syndrome (HUS). Cattle are the main reservoir of these and other Shiga toxin-producing E. coli (STEC).\nDuring this work, protection conferred after immunization of calves with recombinant type-three s...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2390 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2390.dir/2390.PDF |
| Aporte de: |
| Sumario: | Escherichia coli O157:H7 is a zoonotic pathogen and the major etiologic agent of Haemolytic Uremic Syndrome (HUS). Cattle are the main reservoir of these and other Shiga toxin-producing E. coli (STEC).\nDuring this work, protection conferred after immunization of calves with recombinant type-three secreted proteins, the C-terminal fraction of ?-Intimin (?-IntiminC280) and EspB, as well as the B subunit of Shiga toxin 2 fused to Brucella Lumazine Synthase (BLS-Stx2B) was evaluated upon experimental infection. \nImmunization with these antigens induced a significant increase in specific antibodies in sera as well as mucosa, able to neutralize several virulence properties of the bacteria in vitro. After experimental infection, a significant reduction in shedding was observed between vaccinated and control animals. However, no difference was observed between animals vaccinates only with ?-IntiminC280 and EspB, and those vaccinated with these two antigens and BLS-Stx2B.\nThese results suggest that the inclusion of BLS-Stx2B does not confer further protection that the already provided by ?-IntiminC280 and EspB, at least after the first weeks after experimental infection. Further studies are required to elucidate if the addition of this antigen influences E. coli O157:H7 under natural conditions of exposure. |
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