Mecanismos moleculares asociados a la entrada de HCO3 - durante la capacitación espermática humana
Mammalian sperm require to spend a limited period of time in the female reproductive tract to become\ncompetent to fertilize an egg in a process called capacitation. This process consists in a series of cellular\n(hyperactivated motility (HA) and acrosomal exocytosis (AE)) and biochemical changes. A...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2018
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2167 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2167.dir/2167.PDF |
| Aporte de: |
| Sumario: | Mammalian sperm require to spend a limited period of time in the female reproductive tract to become\ncompetent to fertilize an egg in a process called capacitation. This process consists in a series of cellular\n(hyperactivated motility (HA) and acrosomal exocytosis (AE)) and biochemical changes. Among\nbiochemical changes, HCO3\n- influx and the consequent activation of PKA, the increase in intracellular\npH and membrane potential (Em) hyperpolarization are explored in this thesis work. We have analyzed\nhow HCO3\n- is transported into human sperm. We propose that in the seminal plasma there is a\nrapid HCO3\n- influx through NBC cotransporters. As a consequence, this uptake induce an increase in\nthe basal PKA activity. In contrast, the presence of seminal plasma proteins (?PS) modulates the activation\nof PKA. Once in the female tract, there is a removal of ?PS and a sustained HCO3\n- influx mediated\nby CFTR through SLC26. The sustained activation of PKA then modulates changes in pH and\nEm, which are essential for HA and AE. |
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