Estudio de los mecanismos que regulan la activación de linfocitos Tyd inducida por diferentes agonistas : caracterización del rol inmunomodulador de los neutrófilos
In this study, we evaluated the activation of human blood yd T cells stimulated by anti-CD3 antibodies, phosphoantigens and monosodium urate crystals (MSU). Also we investigated the role of neutrophils during the activation of yd T cells. We found that the up-regulation of CD69 expression, and the p...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2017
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_2032 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_2032.dir/2032.PDF |
| Aporte de: |
| Sumario: | In this study, we evaluated the activation of human blood yd T cells stimulated by anti-CD3 antibodies, phosphoantigens and monosodium urate crystals (MSU). Also we investigated the role of neutrophils during the activation of yd T cells. We found that the up-regulation of CD69 expression, and the production of IFN-y and TNF-a induced by anti-CD3 antibodies were potentiated by neutrophils. The inhibition of serine proteases prevented the potentiation of yd T cell activation induced by neutrophils. Moreover, the addition of elastase, cathepsin G and proteinase 3 to yd T cell culture increased their stimulation. We demonstrated that the effect of serine proteases on yd T cells was mediated through the proteases-activated receptor, PAR1. On the other hand, this work demonstrated that MSU crystals activated yd T cells by increasing the expression of CD25 and CD69, and the production of IFN-y and TNF-a. The stimulatory effect of MSU crystals was dependent on Spleen tyrosine kinase Syk, since the treatment of cells with the inhibitor GS-9973 decreased the stimulation of yd T cells. Moreover, the treatment of cells with MSU crystals triggered the phosphorylation of Syk. Also we observed that neutrophils exerted an inhibitory effect on yd T cells activated by MSU crystals. |
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