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Bovine herpesvirus 1 is an alfaherpesvirus that infects cattle producing respiratory, genital and reproductive disease. It is a neurotropic virus that establishes latency in sensory ganglia, and\nviral reexcretion after reactivation of latent infections is responsible for the perpetuation of the\nag...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2010
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1574 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1574.dir/1574.PDF |
| Aporte de: |
| Sumario: | Bovine herpesvirus 1 is an alfaherpesvirus that infects cattle producing respiratory, genital and reproductive disease. It is a neurotropic virus that establishes latency in sensory ganglia, and\nviral reexcretion after reactivation of latent infections is responsible for the perpetuation of the\nagent in the field. In our country, seroprevalence can reach 80% and vaccination is the strategy used to control this disease. A recombinant virus was developed in our laboratory to be used as a marker vaccine, from BoHV-1.1 strain Los Angeles (LA) through allelic replacement of the gene encoding glycoprotein E by the lac z gene, named BoHV-1?gE?gal. Genomic characterization of BoHV-1?gE?gal strain verified the absence of changes in the\ngE gene flanking regions, and specifically identified the sites where allelic exchange occurred. The RFLP study showed that this strain has a different pattern than expected, indicating changes in certain restriction sites. The study of the in vitro behavior established that this strain is less\nefficient in producing infectious viral particles as well as in viral dispersion when compared to the\nparental or other gE gene deleted strains, both in the mechanism of cell-to-cell spread and in the\nrelease of infectious viral particles into the extracellular medium. BoHV-1?gE?gal virus is\nattenuated in the natural host, and this attenuation is greater than that described for other gE gene deleted strains, albeit immunogenic, it is not excreted nor produces clinical disease after intranasal infection, and does not establish a latent infection or is reactivated experimentally. The experience of BoHV-1 viral infection and the methodology developed in this work applied to the analysis of samples from infected animals are a valuable tool for studying the\npathogenesis of genetically modified viruses, recombinant virus and field isolates. On the other\nhand, it is a contribution to the knowledge of parental BoHV-1 LA strain which is used in viral\nchallenge experiences. The results of this thesis work, taken together with the history of immunogenicity, protection against viral challenge and safety of BoHV-1?gE?gal strain and the increasing global trend towards the use of live marker vaccines in controlling diseases caused by BoHV-1, indicate that BoHV-1?gE?gal strain could be incorporated as a live immunogen in vaccination schemes of future BoHV-1 control and eradication plans in our country without risk of\ncausing illness, latency or virus spread to other animals, therefore reducing the incidence of bovine respiratory disease.\n |
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