1567
Toxocariasis is one of the most prevalent parasitic diseases in dogs and cats, worldwide. Toxocara cati is a nematode that afects the digestive tract of cats. Produce eggs that are eliminated to the environment with the feces of their hosts and developed inside an infective larvae for another cats,...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2012
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1567 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1567.dir/1567.PDF |
| Aporte de: |
| Sumario: | Toxocariasis is one of the most prevalent parasitic diseases in dogs and cats, worldwide. Toxocara cati is a nematode that afects the digestive tract of cats. Produce eggs that are eliminated to the environment with the feces of their hosts and developed inside an infective larvae for another cats, paratenic hosts and men. The larvae can migrate through the hosts tissues and produce in man the Larva Migrans syndrome. There is little evidence of biological and immunological behavior of the host against infection by T. cati. Mice are natural paratenic hosts in the parasite's life cycle and larvae remained infective in their tissues for feline hunters. As experimental model, the mouse has similarities with humans both in the migratory route and in the pathogenesis of the disease. The excretory-secretory antigens (ES) released by the parasitic larvae during the migration process play an important role in the diagnosis of human Toxocariasis, the use of immunoglobulins showed the presence of specific infection by ELISA and Western Blot. An experimental study was conducted in mice, with three independent experiments and different doses of larvae of T. cati eggs. There were conducted the study of the migratory pattern and lesions made during the acute and chronic phase of the infection. The immune response was determined by detection of specific immunoglobulins M and G. The larvae migrate to the liver, lung and brain in the acute phase and concentrated in the muscles of the mouse in the chronic stage. Gross lesions were observed on the surface of the brain and lungs, in the acute stage, and microscopically, inflammatory lesions were observed in the liver, lungs, brain and muscle in both stages. There was no granuloma formation or signs of parasitic larval encapsulation during the infection. Significant differences in the behavior of the curves of immunoglobulin M and G, between the infected group and the control group and between p.i. dates\nwere observed, and could be differentiated between acute and chronic infection. Evidence of bands of low molecular weight (31 and 52 kDa) in Western blotting of positive sera by ELISA technique, are indicative of a specific response to Toxocara spp. Infection and differed between inmunoglobulins between acute and chronic phases. IgM bands were observed with greater intensity than IgG in the acute stage, the latter were present in every p.i. date. The results of this study demonstrate the infectivity of T. cati eggs eliminated in cats feces to the environment. The migratory behavior of T. cati larvae in the mouse and its concentration in muscle, reinforce the importance of mouse as paratenic host and reservoir of the parasite cycle in nature.\nThe presence of inflammatory lesions in tissues, in the absence of parasitic granulomas might suggest a lower virulence of this species with similar pathogenic like T. canis. This could lead to reduced immunological restriction of the larvae. The behavior of the immunology of mouse against migration larvae of T. cati showed a tendency that cauld be explained in the acute and chronic stages of migration, indicating a specific response against this species in the low molecular weight bands observed in Western blot positive sera. The results of this study provide new insights into the behavior of the larvae of T. cati during migration in the mouse and the immunopathologic response to infection, showing the potential of the antigen obtained from larvae of eggs presents in stool, and brings a new tool for the diagnosis of Toxocariasis in humans. |
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