Variación de los perfiles de polifenoles en Ligaria cuneifolia, su estudio mediante electroforesis capilar y su relación con la actividad farmacológica
Ligaria cuneifolia (R. et P.) Tiegh. -Loranthaceae-is a hemiparasite plant which grows on different host trees. Due to its morphological similarity, it is considered as the natural substitute for the European mistletoe. Herbal drugs are complex matrices in which multiple components acting synergisti...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Facultad de Farmacia y Bioquímica
2016
|
| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1402 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1402.dir/1402.PDF |
| Aporte de: |
| Sumario: | Ligaria cuneifolia (R. et P.) Tiegh. -Loranthaceae-is a hemiparasite plant which grows on different host trees. Due to its morphological similarity, it is considered as the natural substitute for the European mistletoe. Herbal drugs are complex matrices in which multiple components acting synergistically are responsible for the pharmacological activity. In order to provide scientific support to the popular use of L. cuneifolia, a capillary electrophoretic method was developed and validated to build a chromatographic profile or fingerprint that allows the evaluation of different components for extract standardization. A comparison was made with other chromatographic techniques such as TLC and HPLC. Polyphenolic content was determined by spectroscopic methods. Isolation, purification and structural analysis of compounds were performed by chromatographic and spectroscopic methods. Ten analytes were identified, four of which are reported for the first time in L. cuneifolia. Antioxidant capacity was evaluated by spectroscopic methods, autographic assay on TLC, capillary electrophoresis and ex vivo and in vivo assays. The findings may explain the beneficial effects of L. cuneifolia. Capillary electrophoresis proved to be an appropriate tool for the quality control of herbal drugs, for antioxidant monitoring as well as an attractive alternative to traditional chromatographic techniques. |
|---|