Hipoxia/Reoxigenación como posible factor etiológico de preeclampsia : rol de las Acuaporinas placentarias en el proceso de Apoptosis
Increasing evidence suggests that aquaporins (AQPs) may be involved in the\nmovement of water across the plasma membrane during apoptosis. Although\napoptosis of trophoblast is a natural event, it is exacerbated in pathological processes\nas preeclampsia. Intermittent hypoxia is proposed to be a pot...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Facultad de Farmacia y Bioquímica
2016
|
| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1400 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1400.dir/1400.PDF |
| Aporte de: |
| Sumario: | Increasing evidence suggests that aquaporins (AQPs) may be involved in the\nmovement of water across the plasma membrane during apoptosis. Although\napoptosis of trophoblast is a natural event, it is exacerbated in pathological processes\nas preeclampsia. Intermittent hypoxia is proposed to be a potent inducer of placental\napoptosis. In this work, we aimed to determine the role of placental AQPs in\ntrophoblast apoptosis.\nExplants from normal placentas were cultured in normoxia, hypoxia, and\nhypoxia/reoxygenation (H/R), and apoptotic indexes were evaluated in the presence or\nabsence of AQPs blockers. AQP3 and AQP4 expressions were also studied in\npreeclamptic placentas and in the explant model.\nFirst, we found that AQP3 and AQP4 expressions were reduced in preeclamptic\nplacentas and in explants exposed to H/R. We also found that, by sterically blocking\nAQPs activity with HgCl2, DNA degradation, the number of apoptotic nuclei, Bax\nexpression and caspase?3 activity were drastically reduced. The same results were\nobserved after blocking AQP3 with CuSO4. However, TEA and phloretin treatments\nfailed in abrogate apoptosis.\nTherefore, AQPs and in particular, AQP3, may be involved in the apoptosis of\ntrophoblast. Thus, alterations in AQPs expression might disturb the equilibrium of this\norderly process which may cause preeclampsia. |
|---|