Evaluación de proteínas de una isla de patogenicidad y de envolturas bacterianas de Escherichia coli 0157:H7 como vacunas para reducir su incidencia en bovinos
Enterohemorrhagic Escherichia coli (EHEC) is the pathogen responsible for the hemolytic uremic syndrome (HUS). Argentina has the world's highest incidence of HUS in children under five years. The serotype O157:H7 is the most prevalent in patients with HUS. Cattle are the primary reservoir of EH...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | |
| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
| Publicado: |
Facultad de Farmacia y Bioquímica
2015
|
| Materias: | |
| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1386 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1386.dir/1386.PDF |
| Aporte de: |
| Sumario: | Enterohemorrhagic Escherichia coli (EHEC) is the pathogen responsible for the hemolytic uremic syndrome (HUS). Argentina has the world's highest incidence of HUS in children under five years. The serotype O157:H7 is the most prevalent in patients with HUS. Cattle are the primary reservoir of EHEC O157:H7, and the main source of infection for humans. Vaccination of cattle constitutes a pre-slaughter intervention strategy to reduce shedding of EHEC O157:H7. In this work, two vaccines were evaluated: the first comprises recognized colonization factors, Intimin and EspB proteins recombinantly produced; the second consisting of EHEC O157:H7 bacterial envelopes. An experimental inoculation model was developed, then a vaccination test for each vaccine with subsequent challenge was carried out. Our results indicate that systemic immunization with two doses of vaccine with recombinants EspB and Intimin could reduce the colonization and shedding of EHEC O157:H7. Colonization time and magnitude of excretion were also reduced by subcutaneous immunization of calves with O157: H7 bacterial envelopes. Additional trials would be needed to evaluate the efficacy of vaccines in calves under natural conditions. |
|---|