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Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The diagnosis of paratuberculosis is difficult and the current diagnostic tests are suitable for detection of the clinical phase of the infection.\nThe general aim was to c...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Universidad de Buenos Aires. Facultad de Ciencias Veterinarias
2016
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=avaposgra&cl=CL1&d=HWA_1251 https://repositoriouba.sisbi.uba.ar/gsdl/collect/avaposgra/index/assoc/HWA_1251.dir/1251.PDF |
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| Sumario: | Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The diagnosis of paratuberculosis is difficult and the current diagnostic tests are suitable for detection of the clinical phase of the infection.\nThe general aim was to characterize the total and specific (to Map and its proteins) bovine antibodies, and to identify its role in the viability of bacteria. The especial aims were: To detect the effect of the Map infection on the levels of total antibodies. To characterize the specific humoral immune response to Map and its proteins in naturally- and experimentally-infected bovines. To evaluate the role of specific bovine antibodies to Map and its proteins in the viability of ingested bacteria.\nBovine antibodies were evaluated by ELISA (levels) and by an in vitro macrophages infection model (role). A total of 120 animals were studied. The analyzed groups varied with the experiments: subclinically, clinically and terminally naturally infected, experimentally infected, exposed, immunized with specific proteins of Map and healthy control bovines.\nData demonstrated that:\nMap infection affects the total levels of antibodies in sera and feces samples of animals in the different stages of paratuberculosis: bovines with clinical signs present increased total levels of IgM in sera as well as IgM, IgG and IgA in feces. The study of whole Map or protoplasmic antigen (PPA) specific IgG2 improves the identification of subclinically infected bovines (sensitivity of 54% and 23% to IgG2 and IgG/ELISA-PPA, respectively).\nIgG2 is the first isotype that increases in sera of Map-experimentally infected calves (p=0,013, on 60 days postinfection). Map viability is decreased upon treatment with antibodies from p34r-immunized cattle in the in vitro macrophages infection model. The results impact on the current diagnostic techniques and demonstrate the functional anti-bacterial role of anti-p34r antibodies. These data justify the evaluation of anti-p34r antibodies in infection and protection models |
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