Inquilinus limosus : un reconocimiento de este patógeno emergente
It was conducted the biochemical and molecular study of Inquilinus limosus with clinical samples.\nA cystic fibrosis patient, chronically colonized (2006-2013), showed the presence of a clone with two phenotypic variants, with different levels of biofilm production in in vitro assays.\nHigh concentr...
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| Formato: | Tesis doctoral acceptedVersion |
| Lenguaje: | Español |
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Facultad de Farmacia y Bioquímica
2015
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| Acceso en línea: | http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgraafa&cl=CL1&d=HWA_1158 http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgraafa/index/assoc/HWA_1158.dir/1158.PDF |
| Aporte de: |
| Sumario: | It was conducted the biochemical and molecular study of Inquilinus limosus with clinical samples.\nA cystic fibrosis patient, chronically colonized (2006-2013), showed the presence of a clone with two phenotypic variants, with different levels of biofilm production in in vitro assays.\nHigh concentrations of colistin and tobramycin promoted the biofilm formation with high viability remaining.\nTwo serine-ß-lactamases have been described. INQ-1 and INQ-2, both chromosomal AmpC-type, were showed different affinities for ß-lactam substrates, without regulatory genes and barely affected by classical serine-ß-lactamase inhibitors.\nWith the genome of I. limosus MP06, sequenced by 99.8%, was possible to determine the presence of genes devoted to multidrug-resistance efflux pumps (RND family and ABC Superfamily) and also hypothesized about the pathogenic role of I. limosus assumed by the presence of genes linked with evasion of the host adaptive immune system and intracellular survival.\nThis work has allowed us to characterize I. limosus as an opportunistic pathogen emerging with strategies that allow it to colonize/infect in a recalcitrant way to antimicrobial treatment. |
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