In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action

In this in vitro assay was evaluated: (i) the effect of pH on the bactericidal activity of ciprofloxacin (CFX) against a native strain of Escherichia coli, (ii) the effect of persister bacteria on the concentration-dependent mode of action of CFX. The antibacterial activity of CFX decreased with red...

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Autores principales: PATRICELLI, Paula, DELL'ELCE, Antonella, WEIDMANN, Carolina, RAMIREZ, Eliana, PRESA ROSSA, Candela, AGUIRRE, María S., CADOCHE, Lilian, FORMENTINI, Enrique
Formato: Artículo revista
Lenguaje:Español
Publicado: Universidad Nacional del Litoral 2017
Materias:
pharmacology
Escherichia coli
efficacy
mode of action
pH
persister cells
farmacología
eficacia
modo de acción
bacterias persistentes
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6579
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Biblioteca Virtual - Publicaciones (UNL) de Universidad Nacional del Litoral
id I26-R133-article-6579
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spelling I26-R133-article-65792023-09-15T10:28:02Z In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action Actividad antibacteriana in vitro de Ciprofloxacina sobre una cepa autóctona de Escherichia coli: efecto del pH sobre su potencia y efecto de la persistencia bacteriana sobre su modo de acción PATRICELLI, Paula DELL'ELCE, Antonella WEIDMANN, Carolina RAMIREZ, Eliana PRESA ROSSA, Candela AGUIRRE, María S. CADOCHE, Lilian FORMENTINI, Enrique pharmacology Escherichia coli efficacy mode of action pH persister cells farmacología Escherichia coli eficacia modo de acción pH bacterias persistentes In this in vitro assay was evaluated: (i) the effect of pH on the bactericidal activity of ciprofloxacin (CFX) against a native strain of Escherichia coli, (ii) the effect of persister bacteria on the concentration-dependent mode of action of CFX. The antibacterial activity of CFX decreased with reductions of pH, so the values of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum eradication bacterial concentration (MEBC) were increased when the pH of the culture medium decreased from 7.4 to 6.5 and 5.5. The kinetics of bacterial elimination of CFX presented a biphasic pattern because of the selection of a sub-population of persistent bacteria which presented a slower elimination rate. Therefore, the antibacterial activity of CFX was determined by its concentration in reference to MIC values and the time during which the exposure of the bacteria was maintained. En este trabajo se evaluó in vitro: (i) el efecto del pH sobre la actividad bactericida de ciprofloxacina (CFX) frente a una cepa autóctona de Escherichia coli y (ii) el efecto de las bacterias persistentes sobre el modo de acción concentración dependiente de CFX. La actividad antibacteriana de CFX disminuyó a causa del descenso del pH, por lo que los valores de concentración inhibitoria mínima (CIM), concentración bactericida mínima (CBM) y concentración de erradicación bacteriana mínima (CEBM) se incrementaron cuando el pH del medio de cultivo descendió de 7,4 a valores de 6,5 y 5,5. La cinética de eliminación bacteriana de CFX fue bifásica a causa de la selección de una sub-población de bacterias persistentes que presentaron una velocidad de eliminación más lenta. Por lo tanto la actividad bactericida de CFX fue definida por su concentración en relación a la CIM y el tiempo durante el cual se mantuvo la exposición de las bacterias a ésta.   Universidad Nacional del Litoral 2017-06-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo original Original article application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6579 10.14409/favecv.v15i1/2.6579 FAVE Sección Ciencias Veterinarias; Vol. 15 Núm. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 38-47 FAVE Sección Ciencias Veterinarias; Vol. 15 No. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 38-47 FAVE Sección Ciencias Veterinarias; v. 15 n. 1/2 (2016): FAVE Sección Ciencias Veterinarias; 38-47 2362-5589 1666-938X 10.14409/favecv.v15i1/2 spa https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6579/9603 Derechos de autor 2017 FAVE Sección Ciencias Veterinarias
institution Universidad Nacional del Litoral
institution_str I-26
repository_str R-133
container_title_str Biblioteca Virtual - Publicaciones (UNL)
language Español
format Artículo revista
topic pharmacology
Escherichia coli
efficacy
mode of action
pH
persister cells
farmacología
Escherichia coli
eficacia
modo de acción
pH
bacterias persistentes
spellingShingle pharmacology
Escherichia coli
efficacy
mode of action
pH
persister cells
farmacología
Escherichia coli
eficacia
modo de acción
pH
bacterias persistentes
PATRICELLI, Paula
DELL'ELCE, Antonella
WEIDMANN, Carolina
RAMIREZ, Eliana
PRESA ROSSA, Candela
AGUIRRE, María S.
CADOCHE, Lilian
FORMENTINI, Enrique
In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
topic_facet pharmacology
Escherichia coli
efficacy
mode of action
pH
persister cells
farmacología
Escherichia coli
eficacia
modo de acción
pH
bacterias persistentes
author PATRICELLI, Paula
DELL'ELCE, Antonella
WEIDMANN, Carolina
RAMIREZ, Eliana
PRESA ROSSA, Candela
AGUIRRE, María S.
CADOCHE, Lilian
FORMENTINI, Enrique
author_facet PATRICELLI, Paula
DELL'ELCE, Antonella
WEIDMANN, Carolina
RAMIREZ, Eliana
PRESA ROSSA, Candela
AGUIRRE, María S.
CADOCHE, Lilian
FORMENTINI, Enrique
author_sort PATRICELLI, Paula
title In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
title_short In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
title_full In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
title_fullStr In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
title_full_unstemmed In vitro antibacterial activity of ciprofloxacin against a native strain of Escherichia coli: effect of pH on its potency and effect of bacterial persistence on its mode of action
title_sort in vitro antibacterial activity of ciprofloxacin against a native strain of escherichia coli: effect of ph on its potency and effect of bacterial persistence on its mode of action
description In this in vitro assay was evaluated: (i) the effect of pH on the bactericidal activity of ciprofloxacin (CFX) against a native strain of Escherichia coli, (ii) the effect of persister bacteria on the concentration-dependent mode of action of CFX. The antibacterial activity of CFX decreased with reductions of pH, so the values of minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum eradication bacterial concentration (MEBC) were increased when the pH of the culture medium decreased from 7.4 to 6.5 and 5.5. The kinetics of bacterial elimination of CFX presented a biphasic pattern because of the selection of a sub-population of persistent bacteria which presented a slower elimination rate. Therefore, the antibacterial activity of CFX was determined by its concentration in reference to MIC values and the time during which the exposure of the bacteria was maintained.
publisher Universidad Nacional del Litoral
publishDate 2017
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/6579
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first_indexed 2023-07-05T23:06:53Z
last_indexed 2024-08-13T20:38:24Z
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