Evaluación de la interferencia serológica generadapor las vacunas contra el Complejo Respiratorio Bovinoen el diagnóstico de la Brucelosis bovina
Bovine brucellosis, caused by Brucella abortus, is a zoonotic disease with significant health and economic impact. Its diagnosis relies on serological tests that detect antibodies against the smooth lipopolysaccharide antigen of B. abortus. However, atypical positive serological reactions may occur...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Artículo revista |
| Lenguaje: | Español |
| Publicado: |
Universidad Nacional del Litoral
2026
|
| Materias: | |
| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/index/article/view/15359 |
| Aporte de: |
| Sumario: | Bovine brucellosis, caused by Brucella abortus, is a zoonotic disease with significant health and economic impact. Its diagnosis relies on serological tests that detect antibodies against the smooth lipopolysaccharide antigen of B. abortus. However, atypical positive serological reactions may occur due to infections with other Gram-negative bacteria, vaccination with B. abortus strain 19, or interference from vaccines against the Bovine Respiratory Disease Complex (BRDC). This study evaluated the serological interference of seven commercial BRDC vaccines in the serologic diagnosis of brucellosis. Eight groups of 10 cows were conformed, seven vaccinated and one non-vaccinated control group. Serological tests for brucellosis (BPA, FPA, CFT, and competitive ELISA) were performed from day 0 to day 120 post-vaccination, weekly during the first month and then every 15 days. In addition, PCR for detection of DNA from Brucella spp and sequencing were performed in the vaccines. All groups had BPA-positive animals at some point post-vaccination, with sporadic positive results in complementary tests. One group showed persistent positive reactions in complementary tests, correlated with the detection of Brucella spp DNA in that vaccine, indicating contamination. These findings confirm that BRDC vaccination can interfere with brucellosis diagnosis, highlighting the importance of reviewing vaccination history in cases of atypical results. |
|---|