Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit

It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the tran...

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Autores principales: Guisoni, Nara Cristina, Monteoliva, Diana, Diambra, Luis Aníbal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/86937
Aporte de:
id I19-R120-10915-86937
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Física
Genética
spellingShingle Física
Genética
Guisoni, Nara Cristina
Monteoliva, Diana
Diambra, Luis Aníbal
Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
topic_facet Física
Genética
description It is well known that single-gene circuits with negative feedback loop can lead to oscillatory gene expression when they operate with time delay. In order to generate these oscillations many processes can contribute to properly timing such delay. Here we show that the time delay coming from the transitions between internal states of the cis-regulatory system (CRS) can drive sustained oscillations in an auto-repressive single-gene circuit operating in a small volume like a cell. We found that the cooperative binding of repressor molecules is not mandatory for a oscillatory behavior if there are enough binding sites in the CRS. These oscillations depend on an adequate balance between the CRS kinetic, and the synthesis/ degradation rates of repressor molecules. This finding suggest that the multi-site CRS architecture can play a key role for oscillatory behavior of gene expression. Finally, our results can also help to synthetic biologists on the design of the promoters architecture for new genetic oscillatory circuits.
format Articulo
Articulo
author Guisoni, Nara Cristina
Monteoliva, Diana
Diambra, Luis Aníbal
author_facet Guisoni, Nara Cristina
Monteoliva, Diana
Diambra, Luis Aníbal
author_sort Guisoni, Nara Cristina
title Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
title_short Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
title_full Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
title_fullStr Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
title_full_unstemmed Promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
title_sort promoters architecture-based mechanism for noise-induced oscillations in a single-gene circuit
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/86937
work_keys_str_mv AT guisoninaracristina promotersarchitecturebasedmechanismfornoiseinducedoscillationsinasinglegenecircuit
AT monteolivadiana promotersarchitecturebasedmechanismfornoiseinducedoscillationsinasinglegenecircuit
AT diambraluisanibal promotersarchitecturebasedmechanismfornoiseinducedoscillationsinasinglegenecircuit
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