Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein
<i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host pl...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2013
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/85543 |
| Aporte de: |
| Sumario: | <i>Citrus psorosis virus</i> (CPsV) and <i>Mirafiori lettuce big-vein virus</i> (MiLBVV), members of the <i>Ophioviridae</i> family, have segmented negative-sense single-stranded RNA genomes. To date no reports have described how ophioviruses spread within host plants and/or the proteins involved in this process. Here we show that the 54K protein of CPsV is encoded by RNA 2 and describe its subcellular distribution. Upon transient expression in <i>Nicotiana benthamiana</i> epidermal cells the 54K protein, and also its 54K counterpart protein of MiLBVV, localize to plasmodesmata and enhance GFP cell-to-cell diffusion between cells. Both proteins, but not the coat proteins (CP) of the respective viruses, functionally <i>trans</i>-complement cell-to-cell movement-defective <i>Potato virus</i> X (PVX) and <i>Tobacco mosaic virus</i> (TMV) mutants. The 54K and 54K proteins interact with the virus-specific CP in the cytoplasm, suggesting a potential role of CP in ophiovirus movement. This is the first study characterizing the movement proteins (MP) of ophioviruses. |
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