New validated RP-UPLC method for determination of aripiprazole assay in aripiprazole tablets
A simple, economic and time-efficient, isocratic reverse-phase ultra performance liquid chromatographic (RP-UPLC) method has been developed to analyze aripiprazole in tablets. Successful chromatographic elution and quantification of the drug was achieved on a Waters Symmetry C18, 100 mm x 4.6mm, 3.5...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8410 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_22.pdf |
| Aporte de: |
| Sumario: | A simple, economic and time-efficient, isocratic reverse-phase ultra performance liquid chromatographic (RP-UPLC) method has been developed to analyze aripiprazole in tablets. Successful chromatographic elution and quantification of the drug was achieved on a Waters Symmetry C18, 100 mm x 4.6mm, 3.5 μm column, UV detection at 220 nm with a isocratic mobile phase comprising a mixture of component A (pH 2.5, phosphate buffer) and component B (methanol and acetonitrile (1:1, v/v) in the ratio of 45:55 (v/v). The flow rate was 1.0 mL/min. The method was validated for specificity, precision, linearity, accuracy, range, stability in analytical solution, robustness and system suitability. The linearity concentration range was 5.4-67.8 μg/mL with the correlation coefficient of 0.9997. Total elution time was about 6 min which allowed quantification of more than 100 samples per day. |
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