A high performance liquid chromatography method for determination of hydroxyzine hydrochloride in syrup

A simple isocratic method for quantification of hydroxyzine dihydrochloride by HPLC with UV detection at 232 nm in syrup has been developed and validated. Separation was achieved on a C18 column (250 x 4.6 mm, 5 μm) maintained at 90 ºC with 0.5 mol/L potassium dihydrogen phosphate buffer : acetonitr...

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Detalles Bibliográficos
Autores principales: Ferreira, Anderson O., Grossi, Lívia N., Raposo, Nádia N. B., Vaz, Urias P., Montesano, Gabriela, Fonseca, Roberta G., Polonini, Hudson C., Nascimento, Luiz G.B., Brandão, Marcos A. F.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Estudios de Validación
Cromatografia líquida de alta eficiencia
Hidroxizina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8377
http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_1_21.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
Descripción
Sumario:A simple isocratic method for quantification of hydroxyzine dihydrochloride by HPLC with UV detection at 232 nm in syrup has been developed and validated. Separation was achieved on a C18 column (250 x 4.6 mm, 5 μm) maintained at 90 ºC with 0.5 mol/L potassium dihydrogen phosphate buffer : acetonitrile (1:1, v/v) as mobile phase at a flow rate of 2.0 mL/min. The method was proven to be linear over the range of 80–120 μg/mL, accurate (recovery = 97.8 %), precise (coefficient of variation = 1.52 % for sample) and robust.

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