Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup>
A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The co...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8336 http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_14.pdf |
| Aporte de: |
| Sumario: | A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The contents of the three bioactive constituents (gambogenic acid, R-gambogic acid and S-gambogic) were determined by using HPLC–PDA, and their chemical structures were identified by HPLC-PDA-ESI/MS<sup>n</sup>.The limits of detection and quantitation were between 0.039-0.048 μg/mL and 0.13-0.16 μg/mL. The intra- and inter- assay precisions, in terms of percent relative standard deviation, are less than 3.7 and 4.8 %, respectively . The accuracy, in terms of recovery percentage, ranged from 96.86 to 101.70 %. Good linearity (correlation coefficient > 0.9996) for each calibration curve of standards. HPLC-PDA-ESI/MS<sup>n</sup> was use to analyze caged xanthones in gamboge. A total of 16 peaks were identified or tentatively characterized. The results indicated that the method could be considered to be a simple, rapid and reliable method for the quality evaluation of gamboge. |
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